Ovine echinococcosis: I. Immunological diagnosis by enzyme immunoassay

dc.creatorGatti, Antonio
dc.creatorAlvarez, Angela Rosa
dc.creatorAraya, Daniel
dc.creatorMancini, Sergio
dc.creatorHerrero, Eduardo
dc.creatorSantillan, Graciela
dc.creatorLarrieu, Edmundo
dc.date2021-01-22T19:31:12Z
dc.date2021-01-22T19:31:12Z
dc.date2007-01-31
dc.date.accessioned2023-08-29T20:09:06Z
dc.date.available2023-08-29T20:09:06Z
dc.identifier0304-4017
dc.identifierhttp://sgc.anlis.gob.ar/handle/123456789/2236
dc.identifier10.1016/j.vetpar.2006.08.022
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8520509
dc.descriptionFil: Gatti, Antonio. Ministerio de Salud Provincia de Río Negro; Argentina.
dc.descriptionFil: Alvarez, Angela Rosa. Universidad Nacional de La Pampa. Facultad de Veterinaria; Argentina.
dc.descriptionFil: Araya, Daniel. Universidad Nacional de La Pampa. Facultad de Veterinaria; Argentina.
dc.descriptionFil: Mancini, Sergio. Ministerio de Salud Provincia de Río Negro; Argentina.
dc.descriptionFil: Herrero, Eduardo. Ministerio de Salud Provincia de Río Negro; Argentina.
dc.descriptionFil: Santillan, Graciela. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina.
dc.descriptionFil: Larrieu, Edmundo. Universidad Nacional de La Pampa. Facultad de Veterinaria; Argentina.
dc.descriptionImmunodiagnosis in sheep presents problems of sensitivity and specificity, limiting its applicability in surveillance systems. The objective of this study was to develop a sensitive, specific and accessible technique for diagnosing cystic echinococcosis in naturally infected sheep and to evaluate the validity of necropsy as a reference test. A total of 247 sheep were studied at slaughterhouses, confirming the parasitological diagnosis with histology. Serum was processed with enzyme immunoassay (EIA) using three antigen preparations: total hydatid liquid (LHT), purified fraction of LHT (S2B) and purified lipoprotein (B). Western Blot (WB) was used as a control. EIA proved effective for differentiating Echinococcus granulosus from larval stage of Taenia hydatigena and intestinal cestodes in all three antigen preparations. Serums from macroscopically negative sheep were reactive to EIA and positive with WB. In the whole flock, sensitivity was 89.2% for LHT, 80.0% for S2B and 86.4% for B. Sensitivity in lambs was 78.6% for LHT, 75.0% for S2B and 64.3% for B. Macroscopic diagnosis at the time of slaughter was found to have limitations as a reference test for immunodiagnosis of cystic equinococcosis in sheep, so it was necessary to include histology and WB as reference tests. LHT was the antigen preparation of greatest value and EIA proved to be a sensitive and specific technique, adequate for surveillance systems and for evaluating control programmes.
dc.formatpdf
dc.languageen
dc.relationVeterinary parasitology
dc.rightsnone
dc.sourceVeterinary parasitology 2007;143(2):112-121
dc.subjectEquinococosis
dc.subjectOvinos
dc.subjectPruebas Inmunológicas
dc.subjectTécnicas para Inmunoenzimas
dc.titleOvine echinococcosis: I. Immunological diagnosis by enzyme immunoassay
dc.typeArtículo


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