High polymorphism in genes encoding antigen B from human infecting strains of Echinococcus granulosus

dc.creatorKamenetzky, Laura
dc.creatorMuzulin, Patricia Marcela
dc.creatorGutierrez, Ariana M.
dc.creatorAngel, Sergio O.
dc.creatorZaha, A.
dc.creatorGuarnera, Eduardo
dc.creatorRosenzvit, Mara C.
dc.date2021-01-15T16:39:17Z
dc.date2021-01-15T16:39:17Z
dc.date2005-12
dc.date.accessioned2023-08-29T20:08:46Z
dc.date.available2023-08-29T20:08:46Z
dc.identifier0031-1820
dc.identifierhttp://sgc.anlis.gob.ar/handle/123456789/2148
dc.identifier10.1017/S0031182005008474
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8520337
dc.descriptionFil: Kamenetzky, Laura. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina.
dc.descriptionFil: Muzulin, Patricia Marcela. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina.
dc.descriptionFil: Gutiérrez, Ariana M. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina.
dc.descriptionFil: Angel, Sergio O. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Tecnológico de Chascomús; Argentina.
dc.descriptionFil: Zaha, A. Universidade Federal do Rio Grande do Sul. Instituto de Biociências. Centro de Biotecnologia e Departamento de Biologia Molecular e Biotecnologia; Brasil.
dc.descriptionFil: Guarnera, Eduardo A. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina.
dc.descriptionFil: Rosenzvit, Mara C. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina.
dc.descriptionEchinococcus granulosus antigen B (AgB) is encoded by a gene family and is involved in the evasion of the host immune response. E. granulosus exists as a number of strains (G1-G10) that differ in biological characteristics. We used PCR-SSCP followed by DNA sequencing to evaluate sequence variation and transcription profile of AgB in 5 E. granulosus strains. Twenty-four genomic sequences were isolated and clustered in 3 groups related to 2 of the 5 reported AgB genes. AgB4 genes were present in almost all strains, whereas AgB2 were present as functional genes exclusively in G1/G2 cluster, and as non-functional genes in G5 and the G6/G7 cluster, suggesting inter-strain variation. The AgB transcription patterns, analysed by RT-PCR, showed that AgB2 and AgB4 genes were transcribed in G1, while only the AgB4 gene was transcribed in G7 strain. Cysts from the same strain or cluster shared more genomic and cDNA variants than cysts from different strain or cluster. The level of nucleotide and deduced amino acid sequence variation observed is higher than that reported so far for coding genes of other helminths. Neutrality was rejected for AgB2 genes. These data show the genetic polymorphism of antigen-coding genes among genetically characterized strains of E. granulosus.
dc.languageen
dc.publisherCambridge University Press
dc.relation#PLACEHOLDER_PARENT_METADATA_VALUE#
dc.relationdatasets
dc.relationParasitology
dc.rightsnone
dc.sourceParasitology 2005; 131(6):805-15
dc.subjectSecuencia de Aminoácidos
dc.subjectAnimales
dc.subjectSecuencia de Bases
dc.subjectCamelus
dc.subjectBovinos
dc.subjectEchinococcus granulosus
dc.subjectPerfilación de la Expresión Génica
dc.subjectGenoma de Protozoos
dc.subjectProteínas del Helminto
dc.subjectHumanos
dc.subjectLipoproteínas
dc.subjectDatos de Secuencia Molecular
dc.subjectFilogenia
dc.subjectReacción en Cadena de la Polimerasa
dc.subjectPolimorfismo Genético
dc.subjectPolimorfismo Conformacional Retorcido-Simple
dc.subjectAlineación de Secuencia
dc.subjectAnálisis de Secuencia de ADN
dc.subjectHomología de Secuencia de Aminoácido
dc.subjectHomología de Secuencia de Ácido Nucleico
dc.subjectOvinos
dc.subjectPorcinos
dc.titleHigh polymorphism in genes encoding antigen B from human infecting strains of Echinococcus granulosus
dc.typeArtículo


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