Geographically distinct Escherichia coli O157 isolates differ by lineage, Shiga toxin genotype, and total shiga toxin production

dc.creatorMellor, Glen E
dc.creatorFegan, Narelle
dc.creatorGobius, Kari
dc.creatorSmith, Helen V
dc.creatorJennison, Amy V
dc.creatorD'Astek, Beatriz A
dc.creatorRivas, Marta
dc.creatorShringi, Smriti
dc.creatorBaker, Katherine N K
dc.creatorBesser, Thomas E
dc.date2021-01-05T21:25:24Z
dc.date2021-01-05T21:25:24Z
dc.date2015-02
dc.date.accessioned2023-08-29T20:08:24Z
dc.date.available2023-08-29T20:08:24Z
dc.identifierhttp://sgc.anlis.gob.ar/handle/123456789/2053
dc.identifier10.1128/JCM.01532-14
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8520145
dc.descriptionFil: Mellor, Glen E. Commonwealth Scientific and Industrial Research Organisation. Food and Nutrition Flagship; Australia.
dc.descriptionFil: Fegan, Narelle. Commonwealth Scientific and Industrial Research Organisation. Food and Nutrition Flagship; Australia.
dc.descriptionFil: Gobius, Kari S. Commonwealth Scientific and Industrial Research Organisation. Food and Nutrition Flagship; Australia.
dc.descriptionFil: Smith, Helen V. Forensic and Scientific Services. Department of Health; Australia.
dc.descriptionFil: Jennison, Amy V. Forensic and Scientific Services. Department of Health; Australia.
dc.descriptionFil: D'Astek, Beatriz A. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Servicio Fisiopatogenia; Argentina.
dc.descriptionFil: Rivas, Marta. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Servicio Fisiopatogenia; Argentina.
dc.descriptionFil: Shringi, Smriti. Washington State University. Department of Veterinary Microbiology and Pathology; Estados Unidos.
dc.descriptionFil: Baker, Katherine N K. Washington State University. Department of Veterinary Microbiology and Pathology; Estados Unidos.
dc.descriptionFil: Besser, Thomas E. Washington State University. Department of Veterinary Microbiology and Pathology; Estados Unidos.
dc.descriptionWhile the differential association of Escherichia coli O157 genotypes with animal and human hosts has recently been well documented, little is known about their distribution between countries and how this might affect regional disease rates. Here, we used a 48-plex single nucleotide polymorphism (SNP) assay to segregate 148 E. coli O157 isolates from Australia, Argentina, and the United States into 11 SNP lineages. We also investigated the relationship between SNP lineages, Shiga toxin (Stx) gene profiles, and total Stx production. E. coli O157 isolates clearly segregated into SNP lineages that were differentially associated with each country. Of the 11 SNP lineages, seven were detected among isolates from a single country, two were detected among isolates from all three countries, and another two were detected only among U.S. and Argentinean isolates. A number of Australian (30%) and Argentinean (14%) isolates were associated with novel, previously undescribed SNP lineages that were unique to each country. Isolates within SNP lineages that were strongly associated with the carriage of stx2a produced comparatively more Stx on average than did those lacking the stx2a subtype. Furthermore, the proportion of isolates in stx2a-associated SNP lineages was significantly higher in Argentina and the United States than Australia (P < 0.05). This study provides evidence for the geographic divergence of E. coli O157 and for a prominent role of stx2a in total Stx production. These results also highlight the need for more comprehensive studies of the global distribution of E. coli O157 lineages and the impacts of regionally predominant E. coli O157 lineages on the prevalence and severity of disease.
dc.formatpdf
dc.languageen
dc.publisherAmerican Society for Microbiology
dc.relation#PLACEHOLDER_PARENT_METADATA_VALUE#
dc.relationdatasets
dc.relationJournal of clinical microbiology
dc.rightsopen
dc.sourceJournal of Clinical Microbiology 2015; 53(2):579-586
dc.subjectAnimales
dc.subjectArgentina
dc.subjectAustralia
dc.subjectBovinos
dc.subjectEnfermedades de los Bovinos
dc.subjectInfecciones por Escherichia coli
dc.subjectEscherichia coli O157
dc.subjectHumanos
dc.subjectEpidemiología Molecular
dc.subjectPolimorfismo de Nucleótido Simple
dc.subjectToxina Shiga
dc.subjectEstados Unidos
dc.subjectVariación Genética
dc.subjectGenotipo
dc.subjectFilogeografía
dc.titleGeographically distinct Escherichia coli O157 isolates differ by lineage, Shiga toxin genotype, and total shiga toxin production
dc.typeArtículo


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