Resolving lineage assignation on Mycobacterium tuberculosis clinical isolates classified by spoligotyping with a new high-throughput 3R SNPs based method

dc.creatorAbadia, Edgar
dc.creatorZhang, Jian
dc.creatordos Vultos, Tiago
dc.creatorRitacco, Viviana
dc.creatorKremer, Kristin
dc.creatorAktas, Elif
dc.creatorMatsumoto, Tomoshige
dc.creatorRefregier, Guislaine
dc.creatorvan Soolingen, Dick
dc.creatorGicquel, Brigitte
dc.creatorSola, Christophe
dc.date2021-01-05T21:23:25Z
dc.date2021-01-05T21:23:25Z
dc.date2010-10
dc.date.accessioned2023-08-29T20:08:22Z
dc.date.available2023-08-29T20:08:22Z
dc.identifier1567-1348
dc.identifierhttps://www.sciencedirect.com/science/article/abs/pii/S1567134810001942?via%3Dihub
dc.identifierhttp://sgc.anlis.gob.ar/handle/123456789/2046
dc.identifier10.1016/j.meegid.2010.07.006
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8520131
dc.descriptionFil: Abadia, Edgar. Instituto de Genética y Microbiología, UMR8621, Equipo IGEPE, Universud, CNRS Université; Francia.
dc.descriptionFil: Zhang, Jian. Instituto de Genética y Microbiología, UMR8621, Equipo IGEPE, Universud, CNRS Université; Francia.
dc.descriptionFil: dos Vultos, Tiago. Unité de Génétique Mycobactérienne, Institut Pasteur, París; Francia.
dc.descriptionFil: Ritacco, Viviana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
dc.descriptionFil: Kremer, Kristin. Instituto Nacional de Salud Pública y Medio Ambiente, Bilthoven; Países Bajos.
dc.descriptionFil: Aktas, Elif. Departamento de Microbiología Clínica, Hospital Universitario Zonguldak Karaelmas, Zonguldak; Turquía.
dc.descriptionFil: Matsumoto, Tomoshige. Organización del Hospital de la Prefectura de Osaka Centro Médico de la Prefectura de Osaka para Enfermedades Alérgicas y Respiratorias; Japón.
dc.descriptionFil: Refregier, Guislaine. Instituto de Genética y Microbiología, UMR8621, Equipo IGEPE, Universud, CNRS Université; Francia.
dc.descriptionFil: van Soolingen, Dick. Instituto Nacional de Salud Pública y Medio Ambiente, Bilthoven; Países Bajos.
dc.descriptionFil: Gicquel, Brigitte. Unité de Génétique Mycobactérienne, Institut Pasteur, París; Francia.
dc.descriptionFil: Sola, Christophe. Instituto de Genética y Microbiología, UMR8621, Equipo IGEPE, Universud, CNRS Université; Francia.
dc.descriptionWe developed a new multiplexed-PCR assay to accurately classify Mycobacterium tuberculosis complex (MTC) isolates at the sublineage level by single nucleotide polymorphisms (SNPs). This method relies on 7 SNPs located in different genes of the MTC strains (recC, rec0, recR, ligB, ligC, alkA, and mgtC). Most of these genes are involved in replication, repair and recombination (3R) functions of M. tuberculosis strains, four of the mutations are synonymous, and thus neutral. Genes were chosen as a first empirical approach to assess the congruence between spoligotyping-based phylogeographical classification and SNP typing. This scheme efficiently classifies most of MTC phylogeographical groups: (1) confirming and identifying new sublineage-specific SNPs, (2) unraveling phylogenetical relationships between spoligotyping-defined MTC sublineages, (3) appropriately assigning sublineages to some spoligotypes and reassigning sublineages to other mis-labeled spoligotype signatures. This study opens the way to a more meaningful taxonomic, evolutionary and epidemiological classification. It also allows evaluation of spoligotype-signature significance towards a more comprehensive understanding of the evolutionary mechanisms of the clustered regularly interspaced short palindromic repeat (CRISPR) locus in MTC.
dc.languageen
dc.publisherElsevier
dc.relationInfection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases
dc.rightsnone
dc.sourceInfection, Genetics and Evolution 2010; 10 (7): 1066-74.
dc.subjectMycobacterium tuberculosis
dc.subjectFilogenia
dc.subjectPolimorfismo de Nucleótido Simple
dc.titleResolving lineage assignation on Mycobacterium tuberculosis clinical isolates classified by spoligotyping with a new high-throughput 3R SNPs based method
dc.typeArtículo


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