Epstein-barr virus (EBV) in healthy carriers: Distribution of genotypes and 30 bp deletion in latent membrane protein-1 (LMP-1) oncogene

dc.creatorCorrea, Rita Mariel
dc.creatorFellner, María Dolores
dc.creatorAlonio, Lidia Virginia
dc.creatorDurand, Karina
dc.creatorTeyssie, Angelica R.
dc.creatorPicconi, María Alejandra
dc.date2021-01-05T21:22:00Z
dc.date2021-01-05T21:22:00Z
dc.date2004-08
dc.date.accessioned2023-08-29T20:08:21Z
dc.date.available2023-08-29T20:08:21Z
dc.identifier0146-6615
dc.identifierhttp://sgc.anlis.gob.ar/handle/123456789/2042
dc.identifier10.1002/jmv.20129
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8520123
dc.descriptionFil: Correa, Rita Mariel. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Servicio Virus Oncogénicos; Argentina.
dc.descriptionFil: Fellner, María Dolores. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Servicio Virus Oncogénicos; Argentina.
dc.descriptionFil: Alonio, Lidia Virginia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Servicio Virus Oncogénicos; Argentina.
dc.descriptionFil: Durand, Karina. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Servicio Virus Oncogénicos; Argentina.
dc.descriptionFil: Teyssié, Angélica R. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Servicio Virus Oncogénicos; Argentina.
dc.descriptionFil: Picconi, María Alejandra. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Servicio Virus Oncogénicos; Argentina.
dc.descriptionThere are two types of Epstein Barr virus (EBV): EBV-1 and EBV-2, distinguished by genomic polymorphism in the genes encoding the nuclear antigens (EBNA-2, -3A, -3B, -3C). Latent membrane protein 1 (LMP-1) is an EBV protein with known oncogenic properties. Different variants had been described; among them, a 30 base pair (bp) deletion (del-LMP-1) had been reported in benign and malignant pathologies, but there is little information about its frequency in healthy populations. The aim of this study was to determine the distribution of the EBV genotypes and the 30 bp deletion frequency, in EBV healthy carriers from Argentina. Analysis of EBNA-3C and LMP-1 genes were done by polymerase chain reaction (PCR) followed by Southern blot hybridization on DNA of peripheral blood mononuclear cells (PBMCs) from blood bank donors. EBV-1 was present in 75.9% of samples, EBV-2 in 14.6%, and co-infections with both types in 6.5%. The deleted LMP-1 variant was found in 7.4% of analyzed samples, corresponding 3.2% to deleted variant alone and 4.2% to co-infections with non-deleted form. The non-deleted variant was found in 64.6% whereas in the remaining 28%, no PCR product was detected. These results showed that EBV-1 was the more prevalent type in healthy carriers of Argentina, similar to reports from others countries. A predominance of the non-deleted LMP-1 variant was observed. The presence of co-infections with both types and variants demonstrated that healthy individuals may also harbor multiple EBV infections.
dc.languageen
dc.publisherWiley
dc.publisherWiley-Liss
dc.relation#PLACEHOLDER_PARENT_METADATA_VALUE#
dc.relationdatasets
dc.relationJournal of medical virology
dc.rightsnone
dc.sourceJournal of Medical Virology 2004; 73(4):583-588
dc.subjectAdolescente
dc.subjectAdulto
dc.subjectAnciano
dc.subjectArgentina
dc.subjectDonantes de Sangre
dc.subjectPortador Sano
dc.subjectInfecciones por Virus de Epstein-Barr
dc.subjectFemenino
dc.subjectVariación Genética
dc.subjectGenotipo
dc.subjectHerpesvirus Humano 4
dc.subjectHumanos
dc.subjectLeucocitos Mononucleares
dc.subjectMasculino
dc.subjectPersona de Mediana Edad
dc.subjectReacción en Cadena de la Polimerasa
dc.subjectPrevalencia
dc.subjectProteínas de la Matriz Viral
dc.subjectEliminación de Secuencia
dc.titleEpstein-barr virus (EBV) in healthy carriers: Distribution of genotypes and 30 bp deletion in latent membrane protein-1 (LMP-1) oncogene
dc.typeArtículo


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