Subtyping of Escherichia coli O157:H7 strains isolated from human infections and healthy cattle in Argentina

dc.creatorD'Astek, Beatriz A
dc.creatorDel Castillo, L. L.
dc.creatorMiliwebsky, Elizabeth
dc.creatorCarbonari, Carolina C
dc.creatorPalladino, Martin P
dc.creatorDeza, Natalia L.
dc.creatorChinen, Isabel
dc.creatorManfredi, Eduardo
dc.creatorLeotta, Gerardo A
dc.creatorMasana, Marcelo
dc.creatorRivas, Marta
dc.date2020-12-28T14:35:23Z
dc.date2020-12-28T14:35:23Z
dc.date2012-05
dc.date.accessioned2023-08-29T20:08:15Z
dc.date.available2023-08-29T20:08:15Z
dc.identifier1556-7125
dc.identifierhttps://www.liebertpub.com/doi/10.1089/fpd.2011.1062?url_ver=Z39.88-2003&rfr_id=ori%3Arid%3Acrossref.org&rfr_dat=cr_pub++0pubmed&
dc.identifierhttp://sgc.anlis.gob.ar/handle/123456789/2008
dc.identifier10.1089/fpd.2011.1062
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8520071
dc.descriptionFil: D'Astek, Beatriz A. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Fisiopatogenia; Argentina.
dc.descriptionFil: Del Castillo, L. L. Instituto Tecnología de Alimentos, Centro de Investigación de Agroindustria, Instituto Nacional de Tecnología Agropecuaria (INTA), Morón; Argentina.
dc.descriptionFil: Miliwebsky, Elizabeth. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Fisiopatogenia; Argentina.
dc.descriptionFil: Carbonari, Claudia C. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Fisiopatogenia; Argentina.
dc.descriptionFil: Palladino, Martin P. Instituto Tecnología de Alimentos, Centro de Investigación de Agroindustria, Instituto Nacional de Tecnología Agropecuaria (INTA), Morón; Argentina.
dc.descriptionFil: Deza, Natalia L. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Fisiopatogenia; Argentina.
dc.descriptionFil: Chinen, Isabel. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Fisiopatogenia; Argentina.
dc.descriptionFil: Manfredi, Eduardo. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Fisiopatogenia; Argentina.
dc.descriptionFil: Leotta, Gerardo A. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Fisiopatogenia; Argentina.
dc.descriptionFil: Masana, Marcelo. Instituto Tecnología de Alimentos, Centro de Investigación de Agroindustria, Instituto Nacional de Tecnología Agropecuaria (INTA), Morón; Argentina.
dc.descriptionFil: Rivas, Marta. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Fisiopatogenia; Argentina.
dc.descriptionShiga toxin-producing Escherichia coli (STEC) cause nonbloody (NBD) and bloody diarrhea (BD), and hemolytic uremic syndrome (HUS). Cattle have been described as their main reservoir. STEC O157:H7 is recognized as the predominant serotype in clinical infections, but much less is known about the dominant subtypes in humans and animals or their genetic relatedness. The aims of this study were to compare the STEC O157 subtypes found in sporadic human infections with those in the bovine reservoir using stx-genotyping, phage typing, and XbaI-pulsed-field gel electrophoresis (PFGE), and correlate the subtypes with the severity of clinical manifestations. The 280 STEC O157:H7 strains collected included in this study were isolated from HUS (n=122), BD (n=69), and NBD (n=30) cases, and healthy carriers (n=5), and from bovines (n=54) in the abattoirs. The stx-genotyping showed that stx₂/stx(2c(vh-a)) was predominant in human (76.1%) and in bovine strains (55.5%), whereas the second more important genotype was stx₂ (20.8%) in human and stx(2c(vh-a)) (16.7%) in cattle strains. In human strains, PT4 (37.6%), PT49 (24.3%), and PT2 (18.6%) were the most frequent PTs (80.5%). In bovine isolates, PT2 (26%), PT39 (16.7%), and PT4 and PT49 (11.1% each) were predominant. By XbaI-PFGE, all 280 strains yielded 148 patterns with 75% similarity, and 169 strains were grouped in 37 clusters. Identical PT-PFGE-stx profile combinations were detected in strains of both origins: PT4-AREXH01.0011-stx₂/stx(2c(vh-a)) (12 humans and one bovine), PT4-AREXH01.0543-stx₂/stx(2c(vh-a)) (one human and four bovines), PT2-AREXH01.0076-stx₂/stx(2c(vh-a)) (one human and four bovines), PT49-AREXH01.0175-stx₂/stx(2c(vh-a)) (seven humans and one bovine), and PT49-AREXH01.0022-stx₂/stx(2c(vh-a)) (seven humans and one bovine). No correlation was found among the stx-genotypes, the phage type, and the clinical symptoms.
dc.languageen
dc.relationFoodborne pathogens and disease
dc.rightsnone
dc.subjectToxina Shiga
dc.subjectEscherichia coli
dc.subjectDiarrea
dc.subjectSíndrome Hemolítico-Urémico
dc.titleSubtyping of Escherichia coli O157:H7 strains isolated from human infections and healthy cattle in Argentina
dc.typeArtículo


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