Genotypic characterization of non-O157 Shiga toxin-producing Escherichia coli in beef abattoirs of Argentina

dc.creatorMasana, Marcelo
dc.creatorD'Astek, Beatriz A
dc.creatorPalladino, Martin P
dc.creatorGalli, Lucía
dc.creatorDel Castillo, L. L.
dc.creatorCarbonari, Carolina C
dc.creatorLeotta, G. A.
dc.creatorVilacoba, E.
dc.creatorIrino, Kinue
dc.creatorRivas, Marta
dc.date2020-12-28T14:35:07Z
dc.date2020-12-28T14:35:07Z
dc.date2011-12
dc.date.accessioned2023-08-29T20:08:15Z
dc.date.available2023-08-29T20:08:15Z
dc.identifierhttp://sgc.anlis.gob.ar/handle/123456789/2007
dc.identifier10.4315/0362-028X.JFP-11-189
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8520069
dc.descriptionFil: Masana, Marcelo O. Instituto Tecnología de Alimentos. Centro de Investigación de Agroindustria. Instituto Nacional de Tecnología Agropecuaria; Argentina.
dc.descriptionFil: D'Astek, Beatriz A. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Servicio Fisiopatogenia; Argentina.
dc.descriptionFil: Palladino, Martin P. Instituto Tecnología de Alimentos. Centro de Investigación de Agroindustria. Instituto Nacional de Tecnología Agropecuaria; Argentina.
dc.descriptionFil: Galli, Lucía. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Servicio Fisiopatogenia; Argentina.
dc.descriptionFil: Del Castillo, L L. Instituto Tecnología de Alimentos. Centro de Investigación de Agroindustria. Instituto Nacional de Tecnología Agropecuaria; Argentina.
dc.descriptionFil: Carbonari, Carolina C. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Servicio Fisiopatogenia; Argentina.
dc.descriptionFil: Leotta, G A. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Servicio Fisiopatogenia; Argentina.
dc.descriptionFil: Vilacoba, E. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Servicio Fisiopatogenia; Argentina.
dc.descriptionFil: Irino, Kinue. Instituto Adolfo Lutz. Seção de Bacteriologia; Brasil.
dc.descriptionFil: Rivas, Marta. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Servicio Fisiopatogenia; Argentina.
dc.descriptionThe non-O157 Shiga toxin-producing Escherichia coli (STEC) contamination in carcasses and feces of 811 bovines in nine beef abattoirs from Argentina was analyzed during a period of 17 months. The feces of 181 (22.3%) bovines were positive for non-O157 STEC, while 73 (9.0%) of the carcasses showed non-O157 STEC contamination. Non-O157 STEC strains isolated from feces (227) and carcasses (80) were characterized. The main serotypes identified were O178:H19, O8:H19, O130:H11, and O113:H21, all of which have produced sporadic cases of hemolytic-uremic syndrome in Argentina and worldwide. Twenty-two (7.2%) strains carried a fully virulent stx/eae/ehxA genotype. Among them, strains of serotypes O103:[H2], O145:NM, and O111:NM represented 4.8% of the isolates. Xba I pulsed-field gel electrophoresis pattern analysis showed 234 different patterns, with 76 strains grouped in 30 clusters. Nine of the clusters grouped strains isolated from feces and from carcasses of the same or different bovines in a lot, while three clusters were comprised of strains distributed in more than one abattoir. Patterns AREXSX01.0157, AREXBX01.0015, and AREXPX01.0013 were identified as 100% compatible with the patterns of one strain isolated from a hemolytic-uremic syndrome case and two strains previously isolated from beef medallions, included in the Argentine PulseNet Database. In this survey, 4.8% (39 of 811) of the bovine carcasses appeared to be contaminated with nonO157 STEC strains potentially capable of producing sporadic human disease, and a lower proportion (0.25%) with strains able to produce outbreaks of severe disease.
dc.languageen
dc.publisherInternational Association for Food Protection
dc.relation#PLACEHOLDER_PARENT_METADATA_VALUE#
dc.relationdatasets
dc.relationJournal of food protection
dc.rightsnone
dc.sourceJournal of Food Protection 2011; 74(12):2008-2017
dc.subjectAnimales
dc.subjectArgentina
dc.subjectBovinos
dc.subjectADN Bacteriano
dc.subjectBrotes de Enfermedades
dc.subjectHeces
dc.subjectFemenino
dc.subjectContaminación de Alimentos
dc.subjectGenotipo
dc.subjectSíndrome Hemolítico-Urémico
dc.subjectHumanos
dc.subjectMasculino
dc.subjectPrevalencia
dc.subjectMedición de Riesgo
dc.subjectSerotipificación
dc.subjectEscherichia coli Shiga-Toxigénica
dc.subjectPiel
dc.subjectMataderos
dc.subjectSeguridad de Productos para el Consumidor
dc.titleGenotypic characterization of non-O157 Shiga toxin-producing Escherichia coli in beef abattoirs of Argentina
dc.typeArtículo


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