Genetic diversity of the Junin virus in Argentina: geographic and temporal patterns

dc.creatorGarcia, Jorge
dc.creatorMorzunov, Sergey P
dc.creatorLevis, Silvana
dc.creatorRowe, Joan
dc.creatorCalderón, Gladys
dc.creatorEnria, Delia
dc.creatorSabattini, Marta S.
dc.creatorBuchmeier, M J
dc.creatorBowen, M D
dc.creatorSt Jeor, S C
dc.date2020-12-28T14:31:59Z
dc.date2020-12-28T14:31:59Z
dc.date2000-06-20
dc.date.accessioned2023-08-29T20:08:14Z
dc.date.available2023-08-29T20:08:14Z
dc.identifier0042-6822
dc.identifierhttp://sgc.anlis.gob.ar/handle/123456789/2003
dc.identifier10.1006/viro.2000.0345
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8520063
dc.descriptionFil: García, Jorge B. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Virales Humanas; Argentina.
dc.descriptionFil: Morzunov, Sergey P. University of Nevada at Reno. Department of Microbiology; Estados Unidos.
dc.descriptionFil: Levis, Silvana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Virales Humanas; Argentina.
dc.descriptionFil: Rowe, Joan. University of Nevada at Reno. Department of Microbiology; Estados Unidos.
dc.descriptionFil: Calderón, Gladys. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Virales Humanas; Argentina.
dc.descriptionFil: Enría, Delia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Virales Humanas; Argentina.
dc.descriptionFil: Sabattini, Marta S. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Virales Humanas; Argentina.
dc.descriptionFil: Buchmeier, M J. Scripps Research Institute. Department of Neuropharmacology; Estados Unidos.
dc.descriptionFil: Bowen, M D. Centers for Disease Control and Prevention. Special Pathogens Branch; Estados Unidos.
dc.descriptionFil: St Jeor, Stephen C. University of Nevada at Reno. Department of Microbiology; Estados Unidos.
dc.descriptionRNA was purified from 39 strains of cell-cultured Junin virus (JUN) from central Argentina, which included both human- and rodent-derived isolates (a total of 26 and 13, respectively), as well as from 2 laboratory JUN strains, XJ Cl3 and XJ #44. JUN-specific primers were used to amplify a 511-nucleotide (nt) fragment of the nucleocapsid protein gene and a 495-nt fragment of the glycoprotein 1 (GP1) gene. Genetic diversity among JUN strains studied was up to 13% at the nt level and up to 9% at the amino acid (aa) level for the GP1 gene and up to 9% (nt) and 4% (aa) for the NP gene. Phylogenetic analyses of both genes revealed three distinct clades. The first clade was composed of the JUN strains from the center of the endemic area and included the majority of JUN strains analyzed in the current study. The second clade contained 4 JUN strains isolated between 1963 and 1971 from Cordoba Province, the western-most edge of the known endemic area. The third clade contained 4 JUN strains that originated from Calomys musculinus trapped in Zarate, the northeastern edge of the known endemic area. Certain JUN sequences, which were obtained from GenBank and identified as XJ, XJ #44, and Candid #1 strains, appeared to form a separate clade. Over 400 nt of the GP1 and GP2 genes were additionally sequenced for 7 JUN strains derived from patients with different clinical presentations and outcomes of Argentine hemorrhagic fever. Analysis of the corresponding aa sequences did not allow us to attribute any particular genetic marker to the changing severity or clinical form of the human disease.
dc.formatpdf
dc.languageen
dc.publisherElsevier
dc.publisherAcademic Press
dc.relation#PLACEHOLDER_PARENT_METADATA_VALUE#
dc.relationdatasets
dc.relationVirology
dc.rightsopen
dc.sourceVirology 2000; 272(1):127-136
dc.subjectAnimales
dc.subjectArgentina
dc.subjectLínea Celular
dc.subjectAnálisis Mutacional de ADN
dc.subjectGenes Virales
dc.subjectVariación Genética
dc.subjectGlicoproteínas
dc.subjectFiebre Hemorrágica Americana
dc.subjectHumanos
dc.subjectVirus Junin
dc.subjectRatones
dc.subjectDatos de Secuencia Molecular
dc.subjectMuridae
dc.subjectMutation
dc.subjectNucleocápside
dc.subjectARN Viral
dc.subjectReacción en Cadena de la Polimerasa de Transcriptasa Inversa
dc.subjectHomología de Secuencia
dc.subjectTime Factors
dc.subjectVirulencia
dc.subjectFilogenia
dc.titleGenetic diversity of the Junin virus in Argentina: geographic and temporal patterns
dc.typeArtículo


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