| dc.creator | Quelle, Liliana S | |
| dc.creator | Corso, Alejandra | |
| dc.creator | Galas, Marcelo F. | |
| dc.creator | Sordelli, Daniel O. | |
| dc.date | 2020-12-23T15:39:57Z | |
| dc.date | 2020-12-23T15:39:57Z | |
| dc.date | 2003-11 | |
| dc.date.accessioned | 2023-08-29T20:08:09Z | |
| dc.date.available | 2023-08-29T20:08:09Z | |
| dc.identifier | 0732-8893 | |
| dc.identifier | http://sgc.anlis.gob.ar/handle/123456789/1985 | |
| dc.identifier | 10.1016/s0732-8893(03)00137-8 | |
| dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/8520027 | |
| dc.description | Fil: Quelle, Liliana S. Departamento de Microbiología, Parasitología e Inmunología, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires; Argentina. | |
| dc.description | Fil: Corso, Alejandra. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina. | |
| dc.description | Fil: Galas, Marcelo F. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina. | |
| dc.description | Fil: Sordelli, Daniel O. Departamento de Microbiología, Parasitología e Inmunología, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires; Argentina. | |
| dc.description | A method based on restriction profile analysis of the STAR repetitive element PCR (STAR-RP PCR) product obtained by digestion with AluI and Tru9I was developed for typing methicillin resistant Staphylococcus aureus (MRSA). We evaluated a well defined collection of MRSA from Argentina, previously characterized by PFGE (pulsed field gel electrophoresis) of chromosomal SmaI digests and hybridization with DNA probes for probes ClaI-mecA and ClaI-Tn554. We comparatively evaluated STAR-RP analysis with other PCR based methods such as Inter IS256-PCR, Rep-MP3 PCR and Coa-RP. The discriminatory power (D) of STAR-RP (0.86) was similar to that of PFGE (0.84) at the type level. Comparable results were obtained with Inter IS256 PCR (0.85) and Rep-MP3 PCR (0.80). A lower value (0.74) was obtained for Coa-RP. An excellent reproducibility (100%) of STAR-RP was observed. Good concordance between STAR-RP and other molecular typing methods was found for MRSA isolates (n = 39). STAR-RP typing showed 87% concordance with mecA::Tn554::PFGE, 87% with Inter IS256 PCR and 71% with Rep-MP3 typing. STAR-RP is suggested as an adequate molecular typing assay for MRSA epidemiologic assessment. | |
| dc.language | en | |
| dc.publisher | Elsevier | |
| dc.relation | Diagnostic microbiology and infectious disease | |
| dc.rights | none | |
| dc.source | Diagnostic Microbiology and Infectious Disease 2003 ; 47(3):455-64. | |
| dc.subject | Staphylococcus aureus | |
| dc.title | STAR gene restriction profile analysis in epidemiological typing of methicillin-resistant Staphylococcus aureus: description of the new method and comparison with other polymerase chain reaction (PCR)-based methods | |
| dc.type | Artículo | |
