Genetic characterization of Vibrio cholerae isolates from Argentina by V. cholerae repeated sequences-polymerase chain reaction

dc.creatorCastañeda, Nancy Claudia
dc.creatorPichel, Mariana
dc.creatorOrman, Betina E.
dc.creatorBinsztein, Norma
dc.creatorRoy, Paul H
dc.creatorCentrón, Daniela
dc.date2020-12-23T15:39:42Z
dc.date2020-12-23T15:39:42Z
dc.date2005-11
dc.date.accessioned2023-08-29T20:08:09Z
dc.date.available2023-08-29T20:08:09Z
dc.identifier0732-8893
dc.identifierhttp://sgc.anlis.gob.ar/handle/123456789/1984
dc.identifier10.1016/j.diagmicrobio.2005.05.008
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8520026
dc.descriptionFil: Castañeda, Nancy Claudia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología; Argentina.
dc.descriptionFil: Pichel, Mariana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología; Argentina.
dc.descriptionFil: Orman, Betina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología, Inmunología y Parasitología; Argentina.
dc.descriptionFil: Binsztein, Norma. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología; Argentina.
dc.descriptionFil: Roy, Paul H. Centre Hospitalier Universitaire de Québec. Centre de Recherche en lnfectiologie; Canadá.
dc.descriptionFil: Centrón, Daniela. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología, Inmunología y Parasitología; Argentina.
dc.descriptionWe have developed a novel typing method based on Vibrio cholerae repeat sequences (VCR) using primers directed out of the VCR sequences. To evaluate the VCR-polymerase chain reaction (PCR) as a typing system, 2 categories, efficacy and efficiency, were analyzed in 69 strains of human and environmental V. cholerae O1 toxigenic and nontoxigenic, and non-O1 strains isolated since 1992-2000 from Argentina. The discriminatory power (0.91), stability (0.95), reproducibility (1), typeability (1), rapidity, accessibility, as well ease of use, indicated that the VCR-PCR method provides an alternative useful tool for molecular epidemiology of V. cholerae. The VCR-PCR of V. cholerae isolates showed 29 patterns, of which pattern 1 represented 68% of the V. cholerae O1 isolates, supporting the hypothesis that a clone with epidemic behavior was responsible for the epidemic in Latin America. These results showed a good correlation and a better epidemiologic analysis when the results were compared in parallel with repetitive extragenic palindromic sequences-PCR. In conclusion, VCR-PCR showed excellent performance as a typing method for cholera surveillance programs.
dc.languageen
dc.publisherElsevier
dc.relation#PLACEHOLDER_PARENT_METADATA_VALUE#
dc.relationdatasets
dc.relationDiagnostic microbiology and infectious disease
dc.rightsnone
dc.sourceDiagnostic Microbiology and Infectious Disease 2005; 53(3):175-83
dc.subjectArgentina
dc.subjectCólera
dc.subjectADN Bacteriano
dc.subjectMicrobiología Ambiental
dc.subjectHumanos
dc.subjectReacción en Cadena de la Polimerasa
dc.subjectSecuencias Repetitivas de Ácidos Nucleicos
dc.subjectVibrio cholerae O1
dc.subjectVibrio cholerae no O1
dc.subjectTécnicas de Tipificación Bacteriana
dc.subjectBrotes de Enfermedades
dc.titleGenetic characterization of Vibrio cholerae isolates from Argentina by V. cholerae repeated sequences-polymerase chain reaction
dc.typeArtículo


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