First genetic characterization of Fasciola hepatica in Argentina by nuclear and mitochondrial gene markers

dc.creatorCarnevale, Silvana
dc.creatorMalandrini, Jorge Bruno
dc.creatorPantano, María Laura
dc.creatorSoria, Claudia Cecilia
dc.creatorRodrigues-Silva, Rosângela
dc.creatorMachado-Silva, José Roberto
dc.creatorVelásquez, Jorge Néstor
dc.creatorKamenetzky, Laura
dc.date2020-12-18T16:03:45Z
dc.date2020-12-18T16:03:45Z
dc.date2017-10-15
dc.date.accessioned2023-08-29T20:08:03Z
dc.date.available2023-08-29T20:08:03Z
dc.identifierhttp://sgc.anlis.gob.ar/handle/123456789/1954
dc.identifier10.1016/j.vetpar.2017.08.006
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8519972
dc.descriptionFil: Carnevale, Silvana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
dc.descriptionFil: Malandrini, Jorge Bruno. Universidad Nacional de Catamarca. Facultad de Ciencias de la Salud; Argentina.
dc.descriptionFil: Pantano, María Laura. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
dc.descriptionFil: Soria, Claudia Cecilia. Universidad Nacional de Catamarca. Facultad de Ciencias de la Salud; Argentina.
dc.descriptionFil: Rodrigues-Silva, Rosângela. Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Helmintos Parasitos de Vertebrados; Brasil.
dc.descriptionFil: Machado-Silva, José Roberto. Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Departamento de Microbiologia, Imunologia e Parasitologia. Laboratório de Helmintologia Romero Lascasas Porto; Brasil.
dc.descriptionFil: Velásquez, Jorge Néstor. Hospital Municipal de Infecciosas Dr. Francisco Javier Muñiz; Argentina.
dc.descriptionFil: Kamenetzky, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Microbiología y Parasitología Médica; Argentina.
dc.descriptionFasciola hepatica is a trematode showing genetic variation among isolates from different regions of the world. The objective of this work was to characterize for the first time F. hepatica isolates circulating in different regions of Argentina. Twenty-two adult flukes were collected from naturally infected bovine livers in different areas from Argentina and used for DNA extraction. We carried out PCR amplification and sequence analysis of the ribosomal internal transcribed spacer 1 (ITS1), mitochondrial nicotinamide adenine dinucleotide dehydrogenase subunits 4 and 5 (nad4 and nad5) and mitochondrial cytochrome c oxidase subunit I (cox1) genes as genetic markers. Phylogenies were reconstructed using maximum parsimony algorithm. A total of 6 haplotypes were found for cox1, 4 haplotypes for nad4 and 3 haplotypes for nad5. The sequenced ITS1 fragment was identical in all samples. The analyzed cox1 gene fragment is the most variable marker and is recommended for future analyses. No geographic association was found in the Argentinean samples.
dc.languageen
dc.publisherElsevier
dc.publisherAmerican Association of Veterinary Parasitologists
dc.publisherEuropean Veterinary Parasitology College
dc.publisherWorld Association for the Advancement of Veterinary Parasitology
dc.relation#PLACEHOLDER_PARENT_METADATA_VALUE#
dc.relationdatasets
dc.relationVeterinary parasitology
dc.rightsnone
dc.sourceVeterinary Parasitology 2017; 245:34-38
dc.subjectFasciola hepatica
dc.subjectCiclooxigenasa 1
dc.subjectAnimales
dc.subjectArgentina
dc.subjectADN de Helmintos
dc.subjectADN Mitocondrial
dc.subjectFilogenia
dc.subjectMarcadores Genéticos
dc.titleFirst genetic characterization of Fasciola hepatica in Argentina by nuclear and mitochondrial gene markers
dc.typeArtículo


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