Aporte de la técnica de PCR en el diagnóstico de Mansonella ozzardi en zonas endémicas de la Argentina
Contribution of the PCR assay to the diagnosis of Mansonella ozzardi in endemic areas of Argentina
| dc.creator | Degese, María Fernanda | |
| dc.creator | Cabrera, Marta Graciela | |
| dc.creator | Krivokapich, Silvio | |
| dc.creator | Irazu, Lucía | |
| dc.creator | Rodriguez, Marcelo | |
| dc.creator | Guarnera, Eduardo | |
| dc.date | 2020-12-18T15:59:52Z | |
| dc.date | 2020-12-18T15:59:52Z | |
| dc.date | 2012 | |
| dc.date.accessioned | 2023-08-29T20:08:00Z | |
| dc.date.available | 2023-08-29T20:08:00Z | |
| dc.identifier | 0325-7541 | |
| dc.identifier | http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-75412012000200008 | |
| dc.identifier | http://sgc.anlis.gob.ar/handle/123456789/1941 | |
| dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/8519944 | |
| dc.description | Fil: Degese, María Fernanda. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina. | |
| dc.description | Fil: Cabrera, Marta Graciela. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina. | |
| dc.description | Fil: Krivokapich, Silvio. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina. | |
| dc.description | Fil: Irazu, Lucia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina. | |
| dc.description | Fil: Rodríguez, Marcelo. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina. | |
| dc.description | Fil: Guarnera, Eduardo. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina. | |
| dc.description | Mansonella ozzardi is a tissue-dwelling parasitic nematode, the causative agent of mansonelliasis in almost all Latin American countries. It has been described along the Argentine Yungas region. The microscopic diagnosis can yield false-negative test results at low microfilaremia levels. The aim of this study was to optimize the molecular diagnostic technique and compare it with the Knott's method and standard blood smear procedures (thin blood films and thick smears) in 92 blood samples of individuals from an endemic area. The PCR technique followed by the sequencing of the amplified product yielded 100 % sensitivity compared to the Knott's test, which is considered a reference method. Seven more cases of this parasitosis could only be identified with the molecular technique. | |
| dc.format | ||
| dc.language | es | |
| dc.relation | Revista Argentina de Microbiología | |
| dc.rights | open | |
| dc.subject | Mansonella | |
| dc.subject | Parasitemia | |
| dc.subject | Reacción en Cadena de la Polimerasa | |
| dc.subject | Alineación de Secuencia | |
| dc.subject | Enfermedades Endémicas | |
| dc.title | Aporte de la técnica de PCR en el diagnóstico de Mansonella ozzardi en zonas endémicas de la Argentina | |
| dc.title | Contribution of the PCR assay to the diagnosis of Mansonella ozzardi in endemic areas of Argentina | |
| dc.type | Artículo |