Epstein-Barr virus load in transplant patients: Early detection of post-transplant lymphoproliferative disorders

Carga de virus Epstein-Barr en pacientes trasplantados: detección temprana de desórdenes linfoproliferativos postrasplante

dc.creatorFellner, María Dolores
dc.creatorDurand, Karina A
dc.creatorSolernou, Veronica
dc.creatorBosaleh, Andrea
dc.creatorBalbarrey, Ziomara
dc.creatorGarcía de Dávila, María T
dc.creatorRodriguez, Marcelo
dc.creatorIrazu, Lucía
dc.creatorAlonio, Lidia V.
dc.creatorPicconi, María Alejandra
dc.date2020-12-10T14:28:34Z
dc.date2020-12-10T14:28:34Z
dc.date2016-04
dc.date.accessioned2023-08-29T20:07:42Z
dc.date.available2023-08-29T20:07:42Z
dc.identifier0325-7541
dc.identifierhttp://sgc.anlis.gob.ar/handle/123456789/1864
dc.identifier10.1016/j.ram.2016.02.006
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8519801
dc.descriptionFil: Fellner, María Dolores. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
dc.descriptionFil: Durand, Karina A. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
dc.descriptionFil: Solernou, Veronica. "Prof. Dr. Juan. P. Garrahan" National Pediatrics Hospital. Pathology Service; Argentina.
dc.descriptionFil: Bosaleh, Andrea. "Prof. Dr. Juan. P. Garrahan" National Pediatrics Hospital. Pathology Service; Argentina.
dc.descriptionFil: Balbarrey, Ziomara. "Prof. Dr. Juan. P. Garrahan" National Pediatrics Hospital. Pathology Service; Argentina.
dc.descriptionFil: García de Dávila, María T. "Prof. Dr. Juan. P. Garrahan" National Pediatrics Hospital. Pathology Service; Argentina.
dc.descriptionFil: Rodríguez, Marcelo. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
dc.descriptionFil: Irazu, Lucía. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
dc.descriptionFil: Alonio, Lidia V. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
dc.descriptionFil: Picconi, María A. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
dc.descriptionHigh levels of circulating EBV load are used as a marker of post-transplant lymphoproliferative disorders (PTLD). There is no consensus regarding the threshold level indicative of an increase in peripheral EBV DNA. The aim of the study was to clinically validate a developed EBV quantification assay for early PTLD detection. Transversal study: paired peripheral blood mononuclear cells (PBMC), plasma and oropharyngeal lymphoid tissue (OLT) from children undergoing a solid organ transplant with (n=58) and without (n=47) PTLD. Retrospective follow-up: 71 paired PBMC and plasma from recipients with (n=6) and without (n=6) PTLD history. EBV load was determined by real-time PCR. The diagnostic ability to detect all PTLD (categories 1-4), advanced PTLD (categories 2-4) or neoplastic PTLD (categories 3 and 4) was estimated by analyzing the test performance at different cut-off values or with a load variation greater than 0.5log units. The higher diagnostic performance for identifying all, advanced or neoplastic PTLD, was achieved with cut-off values of 1.08; 1.60 and 2.47log EBVgEq/10(5) PBMC or 2.30; 2.60; 4.47loggEq/10(5) OLT cells, respectively. EBV DNA detection in plasma showed high specificity but low (all categories) or high (advanced/neoplastic categories) sensitivity for PTLD identification. Diagnostic performance was greater when: (1) a load variation in PBMC or plasma was identified; (2) combining the measure of EBV load in PBMC and plasma. The best diagnostic ability to identify early PTLD stages was achieved by monitoring EBV load in PBMC and plasma simultaneously; an algorithm was proposed.
dc.formatpdf
dc.languageen
dc.publisherAsociación Argentina de Microbiología
dc.relation#PLACEHOLDER_PARENT_METADATA_VALUE#
dc.relationdatasets
dc.relationRevista Argentina de microbiologia
dc.rightsopen
dc.sourceRevista Argentina de Microbiología 2016; 48(2):110-118
dc.subjectCarga viral
dc.subjectLinfoma
dc.subjectReacción en Cadena en Tiempo Real de la Polimerasa
dc.subjectHerpesvirus Humano 4
dc.subjectNiño
dc.subjectPreescolar
dc.subjectEstudios Transversales
dc.subjectADN Viral
dc.subjectDetección Precoz del Cáncer
dc.subjectInfecciones por Virus de Epstein-Barr
dc.subjectEstudios de Seguimiento
dc.subjectHumanos
dc.subjectHuésped Inmunocomprometido
dc.subjectLactante
dc.subjectLeucocitos Mononucleares
dc.subjectTejido Linfoide
dc.subjectLinfoma
dc.subjectTrastornos Linfoproliferativos
dc.subjectComplicaciones Posoperatorias
dc.subjectEstudios Retrospectivos
dc.subjectViremia
dc.subjectTrasplante de Corazón
dc.subjectTrasplante de Riñón
dc.subjectTrasplante de Hígado
dc.titleEpstein-Barr virus load in transplant patients: Early detection of post-transplant lymphoproliferative disorders
dc.titleCarga de virus Epstein-Barr en pacientes trasplantados: detección temprana de desórdenes linfoproliferativos postrasplante
dc.typeArtículo


Este ítem pertenece a la siguiente institución