An effective, simple and low-cost pretreatment for culture clarification in tetanus toxoid production

dc.creatorÁvila, Lucía
dc.creatorCascone, Osvaldo
dc.creatorBiscoglio, Mirtha
dc.creatorFingermann, Matías
dc.date2020-12-10T14:29:28Z
dc.date2020-12-10T14:29:28Z
dc.date2018-09
dc.date.accessioned2023-08-29T20:07:41Z
dc.date.available2023-08-29T20:07:41Z
dc.identifierhttp://sgc.anlis.gob.ar/handle/123456789/1867
dc.identifier10.1080/10826068.2018.1509085
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8519792
dc.descriptionFil: Avila, Lucía. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos; Argentina.
dc.descriptionFil: Cascone, Osvaldo. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos; Argentina.
dc.descriptionFil: Biscoglio, Mirtha. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos; Argentina.
dc.descriptionFil: Fingermann, Matías. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos; Argentina.
dc.descriptionChemically inactivated tetanus toxin (tetanus toxoid, TT), purified from cultures of a virulent Clostridium tetani strain, is the active pharmaceutical ingredient of anti-tetanus vaccines. Culture clarification for TT production and is usually performed by filtration-based techniques. Final clarification of the culture supernatant is achieved by passage through 0.2 µm pore size filtering membranes. Large particles removal (primary clarification) before final filtration (secondary clarification) reduces costs of the overall clarification process. With this aim, chitosan-induced particle aggregation was assessed as an alternative for primary clarification. Three chitosan variants were tested with similar results. Optimal clarification of culture supernatant was achieved by the addition of 8 mg chitosan per l of culture. Extrapolation analysis of filter sizing results indicate that 100 l of chitosan-treated supernatant can be finally filtered with a 0.6 m2 normal filtration cartridge of 0.45 + 0.2 µm pore size. The clarified material is compatible with current standard downstream processing techniques for TT purification. Thus, chitosan-induced particle aggregation is a suitable operation for primary clarification.
dc.languageen
dc.relationPreparative biochemistry & biotechnology
dc.rightsnone
dc.subjectTécnicas de Cultivo de Célula
dc.subjectFiltración
dc.subjectFloculación
dc.subjectToxoide Tetánico
dc.titleAn effective, simple and low-cost pretreatment for culture clarification in tetanus toxoid production
dc.typeArtículo


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