Fil: Santos, Patricia Ester. Hospital Nacional de Pediatría Dr. J.P. Garrahan. Servicio de Microbiología; Argentina.Fil: Córdoba, Susana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.Fil: Carrillo-Muñoz, Alfonso. ACIAM. Departamento Microbiología; España.Fil: Rodero, Laura. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.Fil: Rubeglio, Etelvina. Hospital Nacional de Pediatría Dr. J.P. Garrahan. Servicio de Microbiología; Argentina.Fil: Soria, Marcelo. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Microbiología Agrícola; Argentina.Background: A retrospective study on the epidemiology of fungaemia due to yeasts of medical importance at the Hospital Nacional de Pediatría Prof. Dr. J. Garrahan, Buenos Aires was conducted between September 2001 and September 2003. Objectives: To learn the distribution of yeast species and to evaluate their in vitro antifungal susceptibility profile. Methods: The minimum inhibitory concentration (MIC) was determined according to the CLSI M27-A2 procedure, and time kill curves against amphotericin B were also performed. Results-conclusions: The species isolated were Candida parapsilosis (32.6% of isolates); Candida albicans (26.5%), Candida tropicalis (24.5%), and other yeasts (16.4%). Candida isolates were susceptible to the antifungals evaluated, but amphotericin B-tolerant isolates were detected using time kill curves.