Diversity of human herpesvirus 8 genotypes in patients with AIDS and non-AIDS associated Kaposi's sarcoma, Castleman's disease and primary effusion lymphoma in Argentina

dc.creatorPérez, Celeste Luján
dc.creatorTous, Mónica I
dc.date2020-12-04T09:06:25Z
dc.date2020-12-04T09:06:25Z
dc.date2017-11
dc.date.accessioned2023-08-29T20:07:30Z
dc.date.available2023-08-29T20:07:30Z
dc.identifierhttp://sgc.anlis.gob.ar/handle/123456789/1811
dc.identifier10.1002/jmv.24876
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8519694
dc.descriptionFil: Pérez, Celeste Luján. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
dc.descriptionFil: Tous, Mónica I. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
dc.descriptionHHV-8 genotypes are distributed heterogeneously worldwide. The variable K1 gene and the conserved ORF26E region serve to genotype. The aim of the study was to characterize HHV-8 isolates from patients with AIDS, classical, and iatrogenic KS, primary effusion lymphoma and Castleman's disease and one organ donor from Argentina by analysis of ORFK1 and ORF26E regions. DNA was extracted from fresh or paraffin embedded biopsies, blood, and saliva samples and submitted to HHV-8 PCR. Phylogenetic analyses of ORFK1 showed that subtypes C (C1, C2, and C3), B1 and A (A1, A2, and A3) were present in 70.8%, 16.7%, and 12.5% of cases, respectively. Analyses of ORF26E fragment revealed that most strains (45.8%) were subtype A/C while the remaining fall into K, J, B2, R, and D subtypes. Linkage between ORFK1-ORF26E subtypes corresponded to reported relationships, except for one strain that clustered with B1 (K1 African) and D (ORF26E Asian-Pacific) subtypes. This research reveals predominance of subtype C, a broad spectrum of HHV-8 genotypes and reports the first isolation of the African B genotype in Argentina.
dc.languageen
dc.publisherWiley
dc.publisherWiley-Liss
dc.relation#PLACEHOLDER_PARENT_METADATA_VALUE#
dc.relationdatasets
dc.relationJournal of medical virology
dc.rightsnone
dc.sourceJournal of Medical Virology 2017; 89(11):2020-2028
dc.subjectVIH
dc.subjectHerpesvirus Humano 8
dc.subjectVariación Genética
dc.subjectSíndrome de Inmunodeficiencia Adquirida
dc.subjectAdulto
dc.subjectAnciano
dc.subjectArgentina
dc.subjectEnfermedad de Castleman
dc.subjectADN Viral
dc.subjectEvolución Molecular
dc.subjectFemenino
dc.subjectGenotipo
dc.subjectHumanos
dc.subjectLinfoma de Efusión Primaria
dc.subjectMasculino
dc.subjectPersona de Mediana Edad
dc.subjectFilogenia
dc.subjectReacción en Cadena de la Polimerasa
dc.subjectSaliva
dc.subjectSarcoma de Kaposi
dc.subjectDonantes de Tejidos
dc.titleDiversity of human herpesvirus 8 genotypes in patients with AIDS and non-AIDS associated Kaposi's sarcoma, Castleman's disease and primary effusion lymphoma in Argentina
dc.typeArtículo


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