Addition of the immunostimulatory oligonucleotide IMT504 to a seasonal flu vaccine increases hemagglutinin antibody titers in young adult and elder rats, and expands the anti-hemagglutinin antibody repertoire

dc.creatorMontaner, Alejandro Daniel
dc.creatorDenichilo, Analía
dc.creatorRodríguez, Juan Manuel
dc.creatorFló, Juan
dc.creatorLópez, Ricardo Agustin
dc.creatorPontoriero, Andrea
dc.creatorSavy, Vilma L.
dc.creatorBaumeister, Elsa
dc.creatorFrank, Ronald
dc.creatorZorzopulos, Jorge
dc.creatorElías, Fernanda
dc.date2020-12-02T14:30:29Z
dc.date2020-12-02T14:30:29Z
dc.date2011-08
dc.date.accessioned2023-08-29T20:07:28Z
dc.date.available2023-08-29T20:07:28Z
dc.identifier2159-3345
dc.identifierhttp://sgc.anlis.gob.ar/handle/123456789/1800
dc.identifier10.1089/nat.2011.0284
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8519670
dc.descriptionFil: Montaner, Alejandro Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro de Ciencia y Tecnología "Dr. Cesar Milstein"; Argentina.
dc.descriptionFil: Denichilo, Analía. Fundación Pablo Cassará; Argentina.
dc.descriptionFil: Rodríguez, Juan Manuel. Fundación Pablo Cassará; Argentina.
dc.descriptionFil: Fló, Juan. Immunotech S.A.; Argentina.
dc.descriptionFil: López, Ricardo Agustin. Immunotech S.A.; Argentina.
dc.descriptionFil: Pontoriero, Andrea. ANLIS Dr.C.G.Malbrán. Instituto de Enfermedades Infecciosas; Argentina.
dc.descriptionFil: Savy, Vilma. ANLIS Dr.C.G.Malbrán. Instituto de Enfermedades Infecciosas; Argentina.
dc.descriptionFil: Baumeister, Elsa. ANLIS Dr.C.G.Malbrán. Instituto de Enfermedades Infecciosas; Argentina.
dc.descriptionFil: Frank, Ronald. Helmholtz Centre for Infection Research; Braunschweig, Alemania.
dc.descriptionFil: Zorzopulos, Jorge. Fundación Pablo Cassará; Argentina.
dc.descriptionFil: Elías, Fernanda. Fundación Pablo Cassará; Argentina.
dc.descriptionFlu vaccines are partially protective in infants and elder people. New adjuvants such as immunostimulatory oligonucleotides (ODNs) are strong candidates to solve this problem, because a combination with several antigens has demonstrated effectiveness. Here, we report that IMT504, the prototype of a major class of immunostimulatory ODNs, is a potent adjuvant of the influenza vaccine in young adult and elderly rats. Flu vaccines that use virosomes or whole viral particles as antigens were combined with IMT504 and injected in rats. Young adult and elderly animals vaccinated with IMT504-adjuvated preparations reached antibody titers 20-fold and 15-fold higher than controls, respectively. Antibody titers remained high throughout a 120 day-period. Animals injected with the IMT504-adjuvated vaccine showed expansion of the anti-hemagglutinin antibody repertoire and a significant increase in the antibody titer with hemagglutination inhibition capacity when confronted to viral strains included or not in the vaccine. This indicates that the addition of IMT504 in flu vaccines may contribute to the development of significant cross-protective immune response against shifted or drifted flu strains.
dc.languageen
dc.relationNucleic acid therapeutics
dc.rightsnone
dc.subjectAdyuvantes Inmunológicos
dc.subjectSecuencia de Aminoácidos
dc.subjectAnticuerpos
dc.subjectEnsayo de Inmunoadsorción Enzimática
dc.subjectVacunas contra la Influenza
dc.titleAddition of the immunostimulatory oligonucleotide IMT504 to a seasonal flu vaccine increases hemagglutinin antibody titers in young adult and elder rats, and expands the anti-hemagglutinin antibody repertoire
dc.typeArtículo


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