Trypanosoma cruzi loop-mediated isothermal amplification (Trypanosoma cruzi Loopamp) kit for detection of congenital, acute and Chagas disease reactivation

dc.creatorBesuschio, Susana A
dc.creatorPicado, Albert
dc.creatorMuñoz-Calderón, Arturo
dc.creatorWehrendt, Diana P
dc.creatorFernández, Marisa
dc.creatorBenatar, Alejandro Francisco
dc.creatorDiaz Bello, Zoraida
dc.creatorIrurtia, Cecilia
dc.creatorCruz, Israel
dc.creatorNdung'u, Joseph M
dc.creatorCafferata, Maria Luisa
dc.creatorMontenegro, Graciela
dc.creatorSosa-Estani, Sergio
dc.creatorLucero, Raúl H
dc.creatorAlarcón de Noya, Belkisyole
dc.creatorLonghi, Silvia A
dc.creatorSchijman, Alejandro G.
dc.date2020-11-27T01:39:55Z
dc.date2020-11-27T01:39:55Z
dc.date2020
dc.date.accessioned2023-08-29T20:07:20Z
dc.date.available2023-08-29T20:07:20Z
dc.identifierhttp://sgc.anlis.gob.ar/handle/123456789/1746
dc.identifier10.1371/journal.pntd.0008402
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8519613
dc.descriptionFil: Besuschio, Susana A. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor Torres". Laboratorio de Biología Molecular de la Enfermedad de Chagas; Argentina
dc.descriptionFil: Picado, Albert. Foundation for Innovative New Diagnostics (FIND), Suiza.
dc.descriptionFil: Muñoz-Calderón, Arturo. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor Torres". Laboratorio de Biología Molecular de la Enfermedad de Chagas; Argentina
dc.descriptionFil: Wehrendt, Diana P. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor Torres". Laboratorio de Biología Molecular de la Enfermedad de Chagas; Argentina.
dc.descriptionFil: Fernández, Marisa. Hospital de Enfermedades Infecciosas "Dr. Francisco J. Muñiz"; Argentina.
dc.descriptionFil: Benatar, Alejandro. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor Torres". Laboratorio de Biología Molecular de la Enfermedad de Chagas; Argentina.
dc.descriptionFil: Diaz-Bello, Zoraida. Universidad Central de Venezuela, Caracas. Instituto de Medicina Tropical; Venezuela.
dc.descriptionFil: Irurtia, Cecilia. Hospital Nacional "Profesor Alejandro Posadas"; Argentina.
dc.descriptionFil: Cruz, Israel. Foundation for Innovative New Diagnostics (FIND); Suiza.
dc.descriptionFil: Ndung'u, Joseph M. Foundation for Innovative New Diagnostics (FIND); Suiza.
dc.descriptionFil: Cafferata, María L. Instituto de Efectividad Clínica y Sanitaria - Centro de Investigación en Epidemiología y Salud Pública. Departamento en Salud de la Madre y el Niño; Argentina.
dc.descriptionFil: Montenegro, Graciela. Hospital Nacional "Profesor Alejandro Posadas"; Argentina.
dc.descriptionFil: Sosa Estani, Sergio. ANLIS Dr. C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.
dc.descriptionFil: Lucero, Raúl H. Universidad Nacional de Nordeste. Instituto de Medicina Regional. Área de Biología Molecular; Resistencia, Argentina.
dc.descriptionFil: Alarcón de Noya, Belkisyole. Universidad Central de Venezuela, Caracas. Instituto de Medicina Tropical; Venezuela.
dc.descriptionFil: Longhi, Silvia A. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor Torres". Laboratorio de Biología Molecular de la Enfermedad de Chagas; Argentina.
dc.descriptionFil: Schijman, Alejandro G. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor Torres". Laboratorio de Biología Molecular de la Enfermedad de Chagas; Argentina.
dc.descriptionA Trypanosoma cruzi Loopamp kit was recently developed as a ready-to-use diagnostic method requiring minimal laboratory facilities. We evaluated its diagnostic accuracy for detection of acute Chagas disease (CD) in different epidemiological and clinical scenarios. In this retrospective study, a convenience series of clinical samples (venous blood treated with EDTA or different stabilizer agents, heel-prick blood in filter paper or cerebrospinal fluid samples (CSF)) from 30 infants born to seropositive mothers (13 with congenital CD and 17 noninfected), four recipients of organs from CD donors, six orally-infected cases after consumption of contaminated guava juice and six CD patients coinfected with HIV at risk of CD reactivation (N = 46 patients, 46 blood samples and 1 CSF sample) were tested by T. cruzi Loopamp kit (Tc LAMP) and standardized quantitative real-time PCR (qPCR). T. cruzi Loopamp accuracy was estimated using the case definition in the different groups as a reference. Cohen's kappa coefficient (κ) was applied to measure the agreement between Tc LAMP (index test) and qPCR (reference test). Sensitivity and specificity of T. cruzi Loopamp kit in blood samples from the pooled clinical groups was 93% (95% CI: 77-99) and 100% (95% CI: 80-100) respectively. The agreement between Tc LAMP and qPCR was almost perfect (κ = 0.92, 95% CI: 0.62-1.00). The T. cruzi Loopamp kit was sensitive and specific for detection of T. cruzi infection. It was carried out from DNA extracted from peripheral blood samples (via frozen EDTA blood, guanidine hydrochloride-EDTA blood, DNAgard blood and dried blood spots), as well as in CSF specimens infected with TcI or TcII/V/VI parasite populations. The T. cruzi Loopamp kit appears potentially useful for rapid detection of T. cruzi infection in congenital, acute and CD reactivation due to HIV infection.
dc.formatpdf
dc.languageen
dc.relationPLoS neglected tropical diseases
dc.rightsopen
dc.subjectEnfermedad de Chagas
dc.subjectCoinfección
dc.subjectADN Protozoario
dc.subjectInfecciones por VIH
dc.subjectReceptores de Trasplantes
dc.titleTrypanosoma cruzi loop-mediated isothermal amplification (Trypanosoma cruzi Loopamp) kit for detection of congenital, acute and Chagas disease reactivation
dc.typeArtículo


Este ítem pertenece a la siguiente institución