Critical aspects on traditional antivenom production processes and their optimization by factorial analysis

dc.creatorZurbano, Beatriz N
dc.creatorTavarone, Eugenia
dc.creatorViacava, Belén González
dc.creatorDokmetjian, Christian
dc.creatorCascone, Osvaldo
dc.creatorFingermann, Matías
dc.date2020-11-27T13:30:30Z
dc.date2020-11-27T13:30:30Z
dc.date2020-11
dc.date.accessioned2023-08-29T20:07:20Z
dc.date.available2023-08-29T20:07:20Z
dc.identifier1045-1056
dc.identifierhttp://sgc.anlis.gob.ar/handle/123456789/1763
dc.identifier10.1016/j.biologicals.2020.08.005
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8519610
dc.descriptionFil: Zurbano, Beatriz N. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos; Argentina.
dc.descriptionFil: Tavarone, Eugenia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos; Argentina.
dc.descriptionFil: Viacava, Belén González. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos; Argentina.
dc.descriptionFil: Dokmetjian, José C. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos; Argentina.
dc.descriptionFil: Cascone, Osvaldo. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos; Argentina.
dc.descriptionFil: Fingermann, Matías. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos; Argentina.
dc.descriptionMost antivenoms are produced by techniques developed over 50 years ago, with minor modifications. Herein we revise the core of traditional antivenom production processes aiming to optimize key determinants for both consistent antivenom production and the best balance between F(ab')2 quality and recovery. Factorial design analysis revealed that pepsin digestion of 1:3 saline diluted equine plasma for 60 min under pH: 3.20, 37 °C temperature and a 1:15 pepsin to protein ratio conditions, allowed to achieve maximal IgG to F(ab')2 conversion with minimal protein aggregate formation. Further downstream processing by salting out with ammonium sulfate was also studied by factorial analysis. The influence of ammonium sulfate (AS) concentration, temperature (T) and the albumin to total plasma protein ratio plasma (Alb:P) were assayed, revealing that both AS, T and their interaction have a significant impact in F(ab')2 quality and recovery. Taking into account the existing compromise between F(ab')2 monomer recovery and quality two alternative conditions were selected: 14 g/dl AS at 56 °C and, alternatively 16 g/dl AS at 30 °C. Reasonable yields (42%) and product quality (2.5% of aggregates) without significant changes in production cost of traditional methodologies was achieved under the optimized conditions found.
dc.languageen
dc.relationBiologicals : journal of the International Association of Biological Standardization
dc.rightsnone
dc.subjectAntivenenos
dc.subjectPrecipitación salina
dc.subjectPlasma equino
dc.titleCritical aspects on traditional antivenom production processes and their optimization by factorial analysis
dc.typeArtículo


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