dc.creator | Davel, Graciela Odelsia | |
dc.creator | Mazza, Mariana | |
dc.creator | Refojo, Nicolás | |
dc.creator | Taverna, Constanza Giselle | |
dc.creator | Rivas, María Cristina | |
dc.creator | Fernández, Julián | |
dc.creator | Vivot, Matias Ezequiel | |
dc.creator | Mazza, Marta G | |
dc.creator | Mastromónaco, Gladys M | |
dc.creator | Bueno, Nadia Soledad | |
dc.creator | Molina, Viviana | |
dc.creator | Brero, María Luisa | |
dc.creator | Kornblit, Fernando | |
dc.date | 2020-07-04T23:05:25Z | |
dc.date | 2020-07-04T23:05:25Z | |
dc.date | 2019 | |
dc.date.accessioned | 2023-08-29T20:06:54Z | |
dc.date.available | 2023-08-29T20:06:54Z | |
dc.identifier | 0325-7541 | |
dc.identifier | http://sgc.anlis.gov.ar/handle/123456789/1627 | |
dc.identifier | 10.1016/j.ram.2018.08.001 | |
dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/8519384 | |
dc.description | Fil: Davel, Graciela Odelsia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Micología; Argentina. | |
dc.description | Fil: Mazza, M. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Micología; Argentina | |
dc.description | Fil: Refojo, N. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Micología; Argentina | |
dc.description | Fil: Taverna, C. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Micología; Argentina | |
dc.description | Fil: Rivas, M. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Micología; Argentina | |
dc.description | Fil: Fernandez, J. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Micología; Argentina | |
dc.description | Fil: Vivot, M. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Micología; Argentina | |
dc.description | Fil: Mazza, M. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Centro Nacional de Control de Calidad de Biológicos (CNCCB); Argentina | |
dc.description | Fil: Mastomónaco, G. Instituto Nacional de Tecnología Industrial; Argentina. | |
dc.description | Fil: Bueno, N. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Micología; Argentina | |
dc.description | Fil: Molina, V. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina | |
dc.description | Fil: Brero, M L. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Centro Nacional de Control de Calidad de Biológicos (CNCCB); Argentina | |
dc.description | Fil: Brero, M L. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Centro Nacional de Control de Calidad de Biológicos (CNCCB); Argentina | |
dc.description | Fil: Kornblit F. Instituto Nacional de Tecnología Industrial; Argentina. | |
dc.description | Reference fungal cultures (RFCs) are essential for the internal quality control of laboratories. The production of these cultures requires standardized procedures (IRAM 14950:2016 and ISO 17034:2016 standards) carried out by a recognized and accredited laboratory. The aim of this work was to produce RFC in paper disks of autochthonous strains, characterized by two, homogeneous and stable reference methods traceable at species level. RFC were produced using 14 regional species (7 yeasts and 7 filamentous fungi) from the fungal culture collection (DMic). Paper disks were impregnated with a culture suspension, dried and packed. Homogeneity, viability, identity and purity were verified. Short- and long-term stability at different temperatures and storage times were studied. Characterization of each strain allowed to confirm its identity and to ensure its traceability at international level. Produced batches were homogeneous and stable at -20±5°C for 30 months. This method of production was adequate to produce homogeneous and stable RFC with phenotypic and genotypic characteristics correctly defined and internationally traceable. Standardized procedures were developed for the production of certified RFC that could be transferred to other microorganisms. Providing RFC that represent regional strains allows laboratories to produce more reliable results with a favorable impact on medical diagnosis, the environment or the food industry. | |
dc.format | pdf | |
dc.language | en | |
dc.relation | Revista Argentina de microbiologia | |
dc.rights | open | |
dc.source | Revista Argentina de Microbiología 2019;51(3):214:220 | |
dc.subject | Control de calidad | |
dc.subject | Levaduras | |
dc.subject | Micología | |
dc.title | National production of certified reference fungal cultures | |
dc.type | Artículo | |