National production of certified reference fungal cultures

dc.creatorDavel, Graciela Odelsia
dc.creatorMazza, Mariana
dc.creatorRefojo, Nicolás
dc.creatorTaverna, Constanza Giselle
dc.creatorRivas, María Cristina
dc.creatorFernández, Julián
dc.creatorVivot, Matias Ezequiel
dc.creatorMazza, Marta G
dc.creatorMastromónaco, Gladys M
dc.creatorBueno, Nadia Soledad
dc.creatorMolina, Viviana
dc.creatorBrero, María Luisa
dc.creatorKornblit, Fernando
dc.date2020-07-04T23:05:25Z
dc.date2020-07-04T23:05:25Z
dc.date2019
dc.date.accessioned2023-08-29T20:06:54Z
dc.date.available2023-08-29T20:06:54Z
dc.identifier0325-7541
dc.identifierhttp://sgc.anlis.gov.ar/handle/123456789/1627
dc.identifier10.1016/j.ram.2018.08.001
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8519384
dc.descriptionFil: Davel, Graciela Odelsia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Micología; Argentina.
dc.descriptionFil: Mazza, M. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Micología; Argentina
dc.descriptionFil: Refojo, N. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Micología; Argentina
dc.descriptionFil: Taverna, C. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Micología; Argentina
dc.descriptionFil: Rivas, M. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Micología; Argentina
dc.descriptionFil: Fernandez, J. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Micología; Argentina
dc.descriptionFil: Vivot, M. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Micología; Argentina
dc.descriptionFil: Mazza, M. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Centro Nacional de Control de Calidad de Biológicos (CNCCB); Argentina
dc.descriptionFil: Mastomónaco, G. Instituto Nacional de Tecnología Industrial; Argentina.
dc.descriptionFil: Bueno, N. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Micología; Argentina
dc.descriptionFil: Molina, V. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina
dc.descriptionFil: Brero, M L. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Centro Nacional de Control de Calidad de Biológicos (CNCCB); Argentina
dc.descriptionFil: Brero, M L. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Centro Nacional de Control de Calidad de Biológicos (CNCCB); Argentina
dc.descriptionFil: Kornblit F. Instituto Nacional de Tecnología Industrial; Argentina.
dc.descriptionReference fungal cultures (RFCs) are essential for the internal quality control of laboratories. The production of these cultures requires standardized procedures (IRAM 14950:2016 and ISO 17034:2016 standards) carried out by a recognized and accredited laboratory. The aim of this work was to produce RFC in paper disks of autochthonous strains, characterized by two, homogeneous and stable reference methods traceable at species level. RFC were produced using 14 regional species (7 yeasts and 7 filamentous fungi) from the fungal culture collection (DMic). Paper disks were impregnated with a culture suspension, dried and packed. Homogeneity, viability, identity and purity were verified. Short- and long-term stability at different temperatures and storage times were studied. Characterization of each strain allowed to confirm its identity and to ensure its traceability at international level. Produced batches were homogeneous and stable at -20±5°C for 30 months. This method of production was adequate to produce homogeneous and stable RFC with phenotypic and genotypic characteristics correctly defined and internationally traceable. Standardized procedures were developed for the production of certified RFC that could be transferred to other microorganisms. Providing RFC that represent regional strains allows laboratories to produce more reliable results with a favorable impact on medical diagnosis, the environment or the food industry.
dc.formatpdf
dc.languageen
dc.relationRevista Argentina de microbiologia
dc.rightsopen
dc.sourceRevista Argentina de Microbiología 2019;51(3):214:220
dc.subjectControl de calidad
dc.subjectLevaduras
dc.subjectMicología
dc.titleNational production of certified reference fungal cultures
dc.typeArtículo


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