Artículo
A striking common O-linked N-acetylglucosaminyl moiety between cruzipain and myosin
Registro en:
10.1111/j.1365-3024.2011.01291.x
Autor
Acosta, Diana M
Soprano, Luciana L
Ferrero, Maximiliano R
Landoni, Malena
Esteva, Monica I
Couto, Alicia S
Duschak, Vilma G
Resumen
Fil: Acosta, D. M. ANLIS Dr. C. G. Malbrán. Instituto Nacional de Parasitologia Dr. Mario Fatala Chaben (INP); Argentina. Fil: Soprano, L. L. ANLIS Dr. C. G. Malbrán. Instituto Nacional de Parasitologia Dr. Mario Fatala Chaben (INP); Argentina. Fil: Ferrero, M. ANLIS Dr. C. G. Malbrán. Instituto Nacional de Parasitologia Dr. Mario Fatala Chaben (INP); Argentina. Fil: Landoni, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica. Centro de Investigaciones en Hidratos de Carbono (CIHIDECAR); Argentina. Fil: Esteva, Mónica Inés. ANLIS Dr. C. G. Malbrán. Instituto Nacional de Parasitologia Dr. Mario Fatala Chaben (INP); Argentina. Fil: Couto, A. S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica. Centro de Investigaciones en Hidratos de Carbono (CIHIDECAR); Argentina. Fil: Duschak, V. G. ANLIS Dr. C. G. Malbrán. Instituto Nacional de Parasitologia Dr. Mario Fatala Chaben (INP); Argentina. Single units of O-linked N-acetylglucosamine (GlcNAc), usually components of nuclear and cytoplasmatic proteins, are present at the C-terminal domain of cruzipain (Cz), a lysosomal major antigen from Trypanosoma cruzi. On the other hand, antibodies directed against some self-antigens like myosin are associated with Chagas heart disease. The participation of O-GlcNAc moieties in the molecular antigenicity of Cz was determined using GlcNAc linked to aprotinin by ELISA. The immune cross-reactivity between Cz and myosin is mainly focused in the C-T domain. ELISA inhibition assays using rabbit sera specific for Cz and C-T in conjunction with immune-gold electron microscopy analysis of heart tissues from mice immunized with C-T confronted with polyclonal rabbit sera specific for Cz and C-T prior and after myosin adsorption provided evidence which indicates that O-GlcNAc moieties constitute a common epitope between Cz and either myosin or other cardiac O-GlcNAc-containing proteins, showing a new insight into the molecular immune pathogenesis of Chagas heart disease.