info:eu-repo/semantics/article
Characterization of chitin and chitosan extracted from shrimp shells by two methods
Registro en:
1618-7229
10.1515/epoly.2010.10.1.748
Autor
Gartner Vargas, Carmiña
Peláez Jaramillo, Carlos Alberto
López Osorio, Betty Lucy
Institución
Resumen
ABSTRACT:Shrimp shells from Penaeus Vannamei species were hydrolyzed for
chitin extraction by a chemical and a papain enzymatic method. Composition of
shells was analyzed and their microstructure was characterized before and after
hydrolysis by microscopy. Chitin fibers arrangement in the tissue was preserved
after chemical extraction, but after papain hydrolysis the tissue presented structural
disarrangement indicating that papain reacts indistinctly with peptidic and N-acetyl
linkages. Although chemical purification is very effective, by-products are not
recoverable. Conversely, papain hydrolysis yields partially purified chitosan but
permits aminoacids isolation, which is important in food industry. This method has
other advantages such as low cost and easy accessibility of papain. Chitin and
chitosan were characterized by thermogravimetric analysis, infrared
spectrophotometry and capillary electrophoresis. Degree of N-acetylation (DA) was
determined by cross-polarization magic angle spinning nuclear magnetic
resonance (CPMAS 13CNMR) or potentiometry and crystallinity was measured by X
ray diffraction. COL0002401 COL0007462