info:eu-repo/semantics/article
Dendritic Cells from HIV-1 Infected Individuals are Less Responsive to Toll-like Receptor (TLR) Ligands
Registro en:
Martinson JA, Roman-Gonzalez A, Tenorio AR, Montoya CJ, Gichinga CN, Rugeles MT, Tomai M, Krieg AM, Ghanekar S, Baum LL, Landay AL. Dendritic cells from HIV-1 infected individuals are less responsive to toll-like receptor (TLR) ligands. Cell Immunol. 2007 Nov-Dec;250(1-2):75-84. doi: 10.1016/j.cellimm.2008.01.007.
0008-8749
10.1016/j.cellimm.2008.01.007.
1090-2163
Autor
Martinson, Jeffrey A.
Román González, Alejandro
Tenorio, Allan R.
Montoya Guarín, Carlos Julio
Gichinga, Carolyne N.
Rugeles López, María Teresa
Tomai, Mark
Krieg, Arthur M.
Ghanekar, Smita
Baum, Linda L.
Landay, Alan L.
Institución
Resumen
ABSTRACT: We compared TLR responsiveness in PBMC from HIV-1-infected and uninfected individuals using the TLR agonists: TLR7 (3M-001), TLR8 (3M-002), and TLR7/8 (3M-011). Activation and maturation of plasmacytoid dendritic cells (pDC) were measured by evaluating CD86, CD40, and CD83 expression and myeloid dendritic cell (mDC) activation was measured by evaluating CD40 expression. All agonists tested induced activation and maturation of pDC in PBMC cultures of cells from HIV+ and HIV- individuals. The TLR7 agonist induced significantly less pDC maturation in cells from HIV+ individuals. Quantitative assessment of secreted IFN-alpha and pro-inflammatory cytokines at the single cell level showed that pDC from HIV+ individuals stimulated with TLR7 and TLR7/8 induced IFN-alpha. TLR8 and TLR7/8 agonists induced IL-12 and COX-2 expression in mDC from HIV+ and HIV- individuals. Understanding pDC and mDC activation and maturation in HIV-1 infection could lead to more rational development of immunotherapeutic strategies to stimulate the adaptive immune response to HIV-1. COL0012444
Ítems relacionados
Mostrando ítems relacionados por Título, autor o materia.
-
Association of Toll-like receptor 2Arg 753Gln and Toll-like receptor 1Ile 602Ser single-nucleotide polymorphisms with leptospirosis in anargentine population
Cédola, Maia Tatiana; Chiani, Yosena; Prêtre, Gabriela; Alberdi, Maria Lucrecia; Vanasco, Norma Bibiana; Gomez, Ricardo Martin (Elsevier Science, 2015-06)Toll-like receptor 2 (TLR2), a member of the Toll-like receptor family, plays an50 important role in the recognition of and subsequent immune response activation51 against leptospirosis in humans. The genetic polymorphism ...