info:eu-repo/semantics/article
Effects of sterilization techniques on the Pegylated poly (y-benzyl-L-glutamate) (PBLG) nanoparticles
Autor
Özcan, Ipek
Bouchemal, Kawthar
Segura Sánchez, Freimar
Abacıa, Özlem
Özer, Özgen
Güneri, Tamer
Ponchel, Gilles
Institución
Resumen
ABSTRACT: Nanoparticles were prepared by nanoprecipitation technique using poly (1-benzyl-L-glutamate) (PBLG) derivatives. Injectable nanoparticles were assessed for their in vitro properties such as particle size,
polydispersity index, zeta potential and surface properties. The possibilities of sterilizing PBLG
nanoparticle suspensions by membrane fıltration or autoclaving were evaluated. It was found that among
these techniques, sterilization with membrane filtration seemed to be the most appropriate technique with
any significant effect on in vitro and surface properties. Sterility was assessed on üe final product
according to pharmacopoeial requirements. Sterility testing results showed no microbial contamination
indicating that sterile nanoparticle formulations have been achieved after membrane filtration. COL0065152