Human neutrophils produce extracellular traps against Paracoccidioides brasiliensis

dc.creatorHernández Ruiz, Orville
dc.creatorCano Restrepo, Luz Elena
dc.creatorGonzález Marín, Ángel Augusto
dc.creatorLópez Quintero, Juan Alvaro
dc.creatorMejía de los Ríos, Susana Pamela
dc.date2022-03-10T17:03:19Z
dc.date2022-03-10T17:03:19Z
dc.date2015
dc.date.accessioned2023-08-28T20:21:18Z
dc.date.available2023-08-28T20:21:18Z
dc.identifierMejía SP, Cano LE, López JA, Hernandez O, González Á. Human neutrophils produce extracellular traps against Paracoccidioides brasiliensis. Microbiology (Reading). 2015 May;161(Pt 5):1008-1017. doi: 10.1099/mic.0.000059.
dc.identifier1350-0872
dc.identifierhttp://hdl.handle.net/10495/26500
dc.identifier10.1099/mic.0.000059
dc.identifier1465-2080
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8477853
dc.descriptionABSTRACT : Neutrophils play an important role as effector cells and contribute to the resistance of the host against microbial pathogens. Neutrophils are able to produce extracellular traps (NETs) in response to medically important fungi, including Aspergillus spp., Candida albicans and Cryptococcus gattii. However, NET production in response to Paracoccidioides brasiliensis has yet to be studied. We have demonstrated that human neutrophils produce NETs against both conidia and yeasts of P. brasiliensis. Although the NADPH oxidase inhibitor diphenyleneiodonium chloride (DPI) did not alter NET production against conidia, it partially suppressed NET formation against P. brasiliensis yeasts. Cytochalasin D or IFN-c did not affect the production of NETs against the fungus. Additionally, a mutant strain of P. brasiliensis with reduced expression of an alternative oxidase induced significantly higher levels of NETs in comparison with the WT strain. Finally, c.f.u. quantification of P. brasiliensis showed no significant differences when neutrophils were treated with DPI, DNase I or cytochalasin D as compared with untreated cells. These data establish that NET formation by human neutrophils appears to be either dependent or independent of reactive oxygen species production, correlating with the fungal morphotype used for stimulation. However, this mechanism was ineffective in killing the fungus.
dc.descriptionCOL0126131
dc.descriptionCOL0012426
dc.descriptionCOL0000962
dc.format10
dc.formatapplication/pdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherMicrobiology Society
dc.publisherBiología Celular y Molecular CIB U. de A. U. del Rosario
dc.publisherGrupo de Investigación en Microbiología Básica y Aplicada-Microba
dc.publisherInmunodeficiencias Primarias
dc.publisherReading, Inglaterra
dc.relationMicrobiology (Reading)
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://creativecommons.org/licenses/by/2.5/co/
dc.rightshttp://purl.org/coar/access_right/c_abf2
dc.rightshttps://creativecommons.org/licenses/by/4.0/
dc.subjectTrampas Extracelulares
dc.subjectExtracellular Traps
dc.subjectParacoccidioides
dc.subjectParacoccidioidomicosis
dc.subjectParacoccidioidomycosis
dc.subjectProteínas de Plantas
dc.subjectPlant Proteins
dc.subjectEspecies Reactivas de Oxígeno - metabolismo
dc.subjectReactive Oxygen Species - metabolism
dc.titleHuman neutrophils produce extracellular traps against Paracoccidioides brasiliensis
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typehttp://purl.org/coar/resource_type/c_2df8fbb1
dc.typehttps://purl.org/redcol/resource_type/ART
dc.typeArtículo de investigación


Este ítem pertenece a la siguiente institución