info:eu-repo/semantics/article
Human neutrophils produce extracellular traps against Paracoccidioides brasiliensis
Registro en:
Mejía SP, Cano LE, López JA, Hernandez O, González Á. Human neutrophils produce extracellular traps against Paracoccidioides brasiliensis. Microbiology (Reading). 2015 May;161(Pt 5):1008-1017. doi: 10.1099/mic.0.000059.
1350-0872
10.1099/mic.0.000059
1465-2080
Autor
Hernández Ruiz, Orville
Cano Restrepo, Luz Elena
González Marín, Ángel Augusto
López Quintero, Juan Alvaro
Mejía de los Ríos, Susana Pamela
Institución
Resumen
ABSTRACT : Neutrophils play an important role as effector cells and contribute to the resistance of the host against microbial pathogens. Neutrophils are able to produce extracellular traps (NETs) in
response to medically important fungi, including Aspergillus spp., Candida albicans and
Cryptococcus gattii. However, NET production in response to Paracoccidioides brasiliensis has
yet to be studied. We have demonstrated that human neutrophils produce NETs against both
conidia and yeasts of P. brasiliensis. Although the NADPH oxidase inhibitor diphenyleneiodonium
chloride (DPI) did not alter NET production against conidia, it partially suppressed NET formation
against P. brasiliensis yeasts. Cytochalasin D or IFN-c did not affect the production of NETs
against the fungus. Additionally, a mutant strain of P. brasiliensis with reduced expression of an
alternative oxidase induced significantly higher levels of NETs in comparison with the WT strain.
Finally, c.f.u. quantification of P. brasiliensis showed no significant differences when neutrophils
were treated with DPI, DNase I or cytochalasin D as compared with untreated cells. These data
establish that NET formation by human neutrophils appears to be either dependent or
independent of reactive oxygen species production, correlating with the fungal morphotype used
for stimulation. However, this mechanism was ineffective in killing the fungus. COL0126131 COL0012426 COL0000962