info:eu-repo/semantics/article
Fermented Non-Digestible Fraction of Andean Berry (Vaccinium meridionale Swartz) Juice Induces Apoptosis in Colon Adenocarcinoma Cells
Registro en:
Agudelo CD, Luzardo-Ocampo I, Hernández-Arriaga AM, Rendón JC, Campos-Vega R, Maldonado-Celis ME. Fermented Non-Digestible Fraction of Andean Berry (Vaccinium meridionale Swartz) Juice Induces Apoptosis in Colon Adenocarcinoma Cells. Prev Nutr Food Sci. 2020 Sep 30;25(3):272-279. doi: 10.3746/pnf.2020.25.3.272. PMID: 33083376; PMCID: PMC7541930.
2287-1098
10.3746/pnf.2020.25.3.272
2287-8602
Autor
Agudelo Rivera, Carlos Daniel
Luzardo Ocampo, Iván
Hernández Arriaga, Angélica María
Rendón Londoño, Julio César
Campos Vega, Rocío
Maldonado Celis, María Elena
Institución
Resumen
ABSTRACT: Vaccinium meridionale Swartz, known as Andean berry, has a high content of anthocyanins, phenolic acids, and
other flavonoids due to their putative anticancer activity. However, after consumption, the structures and function of these
molecules may be altered. The purpose of this study was to evaluate the pro-apoptotic effect of fermented non-digestible
fraction (FNDF) of Andean berry juice (ABJ) on colon adenocarcinoma HT29 cells. HT29 cells were treated by FNDF-ABJ
obtained by in vitro gastrointestinal fermentation. We determined the proapoptotic capacity by terminal deoxynucleotidyl
transferase dUTP nick end labeling (TUNEL) assays, oxidative stress by analyzing superoxide dismutase and catalase activity, lipid peroxidation by measuring 8-iso-prostaglandin F2, and measured lactate dehydrogenase. Our results show
that FNDF-ABJ inhibited cell growth [lethal dose 50(%)=26% v/v]. In addition, FNDF-ABJ increased the number of
TUNEL positive cells 2-fold compared with untreated cells without altering the release of lactate dehydrogenase. However,
superoxide dismutase activity was reduced in HT29 cells treated with FNDF-ABJ, catalase activity was not affected and
8-iso-prostaglandin F2 levels were increased. These results support that the anti-proliferative effects of FNDF-ABJ on
HT29 cells can be explained by apoptotic mechanisms. COL0083811