Genomic and Toxigenic Heterogeneity of Bacillus cereus sensu lato Isolated from Ready-to-Eat Foods and Powdered Milk in Day Care Centers in Colombia

dc.creatorSánchez Chica, Jennifer
dc.creatorCorrea Ochoa, Margarita María
dc.creatorAceves Diez, Ángel
dc.creatorRasschaert, Geertrui
dc.creatorHeyndrickx, Marc
dc.creatorCastañeda Sandoval, Laura Margarita
dc.date2022-06-29T20:07:07Z
dc.date2022-06-29T20:07:07Z
dc.date2020
dc.date.accessioned2023-08-28T19:53:15Z
dc.date.available2023-08-28T19:53:15Z
dc.identifierSánchez Chica J, Correa MM, Aceves-Diez AE, Rasschaert G, Heyndrickx M, Castañeda-Sandoval LM. Genomic and Toxigenic Heterogeneity of Bacillus cereus sensu lato Isolated from Ready-to-Eat Foods and Powdered Milk in Day Care Centers in Colombia. Foodborne Pathog Dis. 2020 May;17(5):340-347. doi: 10.1089/fpd.2019.2709.
dc.identifier1535-3141
dc.identifierhttp://hdl.handle.net/10495/29478
dc.identifier10.1089/fpd.2019.2709
dc.identifier1556-7125
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8470282
dc.descriptionABSTRACT : Bacillus cereus sensu lato (s.l.) is a group of bacteria commonly found in diverse environments, including foods, with potential to cause emesis and diarrhea. In Colombia, it is one of the main foodborne pathogens. The aim of this study was to determine the genomic and toxigenic heterogeneity of B. cereus s.l. isolated from ready-to-eat foods and powdered milk collected in day care centers of Medellin, Colombia. Of 112 B. cereus s.l. isolates obtained, 94% were b-hemolytic. Toxigenic heterogeneity was established by the presence of nheABC, hblCDAB, cytK2, entFM, and cesB toxigenic genes. The nheABC operon and entFM gene were most frequently detected in the isolates, whereas the cesB gene was not found. According to the toxin genes content, nine toxigenic profiles were identified. A 44% of isolates had profiles with all genes for nonhemolytic enterotoxin, hemolysin BL, and enterotoxin FM production (profiles II and IV). Pulsed-field gel electrophoresis analysis indicated a high genomic heterogeneity among the B. cereus s.l., with 68 isolates grouping into 16 clusters and 33 placed separately in the dendrogram. This study provides useful information on the safety of ready-to-eat foods and powdered milk in day care centers where children, a susceptible population, are exposed and it should incentive for more studies to understand the distribution of different toxin-encoding genes among B. cereus s.l. isolates, enabling detailed risk assessment.
dc.descriptionCOL0040189
dc.descriptionCOL0013746
dc.format9
dc.formatapplication/pdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherMary Ann Liebert
dc.publisherMicrobiología Ambiental
dc.publisherMicrobiología Molecular
dc.publisherLarchmont, Estados Unidos
dc.relationFoodborne. Pathog. Dis.
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://creativecommons.org/licenses/by-nc/2.5/co/
dc.rightshttp://purl.org/coar/access_right/c_abf2
dc.rightshttps://creativecommons.org/licenses/by-nc/4.0/
dc.subjectBacillus cereus
dc.subjectEnterotoxinas
dc.subjectEnterotoxins
dc.subjectToxinas Bacterianas
dc.subjectBacterial Toxins
dc.subjectGenes Bacterianos
dc.subjectGenes, Bacterial
dc.titleGenomic and Toxigenic Heterogeneity of Bacillus cereus sensu lato Isolated from Ready-to-Eat Foods and Powdered Milk in Day Care Centers in Colombia
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typehttp://purl.org/coar/resource_type/c_2df8fbb1
dc.typehttps://purl.org/redcol/resource_type/ART
dc.typeArtículo de investigación


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