This report provides the guiding framework for cities to transform their relationship with nature and to drive an urban development that leaves natural capital enriched and not depleted, a vision that the Colombian government has defined as “BiodiverCities”. Building on this country’s leadership, the World Economic Forum and Colombia have partnered to scale a global initiative with a major ambition: BiodiverCities by 2030. We have the opportunity to design more resilient and adaptive futures for cities across the globe by drawing together multidisciplinary expertise and spurring nature-positive entrepreneurial approaches in the next decade. We encourage city officials, the private sector and the whole urban community to harvest and spread the evidence on the real opportunities for cities to reverse their impact on nature and to build an enabling environment in their own city contexts whereby biodiversity and nature-based solutions are brought to the centre of the urban agenda.Bogotá D.C.