Splenic autoimplantation in omentum and stomach, hematoimmunological follow-up and B-cell repertoire in the graf

dc.creatorÁlvarez, Fernando Andrés
dc.creatorColombres, Gustavo Adolfo
dc.creatorViqueira, Alejandro
dc.creatorMaldonado, Jorge Eduardo
dc.creatorAlvarellos, Emilio
dc.creatorSambuelli, Rubén Horacio
dc.creatorAlvarellos, Teresita
dc.date2010-12-31
dc.date2023-04-10T17:50:01Z
dc.date2023-04-10T17:50:01Z
dc.date.accessioned2023-08-24T11:52:39Z
dc.date.available2023-08-24T11:52:39Z
dc.identifierhttp://pa.bibdigital.ucc.edu.ar/3443/1/A_%C3%81lvarez_Colombres_Viqueira_Maldonado_Alvarellos_Sambuelli_Alvarellos.pdf
dc.identifierhttps://hdl.handle.net/20.500.12032/85896
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8420558
dc.descriptionSplenic autoimplantation appears to be the only alternative to preserve splenic tissue after splenectomy; however, its relevance is still controversial. We intended to study splenic autoimplantation in the greater omentum and stomach wall of rabbits and analyze its hematoimmunological performance and the preservation of original structures. Methods. New Zealand rabbits were divided in two groups: autoimplanted (A) (n=13) and splenectomized (S) (n=4). The animals of group A underwent autoimplantation of splenic fragments in the greater omentum and gastric wall. Both groups were evaluated by hemocytological tests, scintigraphy, immunoglobulin and C3 dosages, before the surgery and 2 and 4 months afterwards. After 4 months, the grafts were removed and histological examination and gen rearrangement of B-lymphocytes receptors by polymerase chain reaction (PCR) were performed to assess the cellular diversity of clones. Results. The histological analysis demonstrated the presence of splenic tissue in 10 of the 13 cases (77%) with evident size reduction. The gastric location did not develop complications and demonstrated higher morphological correspondence to the autoimplanted tissue. Both groups showed significant decrease of IgM and increase of C3, without considerable differences between both of them during follow up. From the 8 grafts studied with PCR, 3 cases presented polyclonality and 5 oligoclonality. Conclusions. The revascularized grafts evidenced splenic regenerating tissue, probably associated to the oligoclonality detected by PCR. Consequently, we consider that autoimplantation is a reasonable alternative for splenectomized patients, even though the stomach placement and the high frequency of oligoclonality justify further investigation.
dc.descriptionFil: Álvarez, Fernando Andrés. Universidad Católica de Córdoba. Facultad de Ciencias de la Salud; Argentina
dc.descriptionFil: Colombres, Gustavo Adolfo. Universidad Católica de Córdoba. Facultad de Ciencias de la Salud; Argentina
dc.descriptionFil: Viqueira, Alejandro. Universidad Católica de Córdoba. Facultad de Ciencias de la Salud; Argentina
dc.descriptionFil: Maldonado, Jorge Eduardo. Universidad Católica de Córdoba. Facultad de Ciencias de la Salud; Argentina
dc.descriptionFil: Alvarellos, Emilio. Universidad Católica de Córdoba. Facultad de Ciencias de la Salud; Argentina
dc.descriptionFil: Sambuelli, Rubén Horacio. Universidad Católica de Córdoba. Facultad de Ciencias de la Salud; Argentina
dc.descriptionFil: Alvarellos, Teresita. Universidad Católica de Córdoba. Facultad de Ciencias de la Salud; Argentina
dc.formatapplication/pdf
dc.languagespa
dc.publisherSociedad Argentina de Gastroenterología
dc.relationhttp://pa.bibdigital.ucc.edu.ar/3443/
dc.relationhttps://www.redalyc.org/articulo.oa?id=199317295009
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.sourceÁlvarez, Fernando Andrés ORCID: https://orcid.org/0000-0003-4644-5665 <https://orcid.org/0000-0003-4644-5665>, Colombres, Gustavo Adolfo, Viqueira, Alejandro ORCID: https://orcid.org/0000-0001-7685-8853 <https://orcid.org/0000-0001-7685-8853>, Maldonado, Jorge Eduardo, Alvarellos, Emilio, Sambuelli, Rubén Horacio and Alvarellos, Teresita ORCID: https://orcid.org/0000-0002-2618-7913 <https://orcid.org/0000-0002-2618-7913> (2010) Splenic autoimplantation in omentum and stomach, hematoimmunological follow-up and B-cell repertoire in the graf. Acta Gastroenterológica Latinoamericana, 40 (4). pp. 339-346. ISSN 0300-9033
dc.subjectR Medicina (General)
dc.titleSplenic autoimplantation in omentum and stomach, hematoimmunological follow-up and B-cell repertoire in the graf
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/acceptedVersion


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