Exposure to repeated immobilization stress inhibits cocaine-induced increase in dopamine extracellular levels in the rat ventral tegmental area

Pharmacological Research

dc.creatorSotomayor-Zárate, Ramón
dc.creatorAbarca, Jorge
dc.creatorAraya, Katherine A
dc.creatorRenard, Georgina M
dc.creatorAndrés, María E
dc.creatorGysling-Caselli, Katia Cecilia
dc.date2021-08-23T22:58:55Z
dc.date2022-07-07T14:55:45Z
dc.date2021-08-23T22:58:55Z
dc.date2022-07-07T14:55:45Z
dc.date2015
dc.date.accessioned2023-08-23T00:32:16Z
dc.date.available2023-08-23T00:32:16Z
dc.identifier1150244
dc.identifier1150244
dc.identifierhttps://hdl.handle.net/10533/252448
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8355515
dc.descriptionA higher vulnerability to drug abuse has been observed in human studies of individuals exposed to chronic or persistent stress, as well as in animal models of drug abuse. Here, we explored the effect of repeated immobilization stress on cocaine-induced increase in dopamine extracellular levels in VTA and its regulation by corticotropin-releasing factor (CRF) and GABA systems. Cocaine (10 mg/Kg i.p.) induced an increase of VTA DA extracellular levels in control rats. However, this effect was not observed in repeated stress rats. Considering the evidence relating stress with CRF, we decided to perfuse CRF and CP-154526 (selective antagonist of CRF1 receptor) in the VTA of control and repeated stress rats, respectively. We observed that perfusion of 20 mu M CRF inhibited the increase of VTA DA extracellular levels induced by cocaine in control rats. Interestingly, we observed that in the presence of 10 mu M CP-154526, cocaine induced a significant increase of VTA DA extracellular levels in repeated stress rats. Regarding the role of VTA GABA neurotransmission, cocaine administration induced a significant increase in VTA GABA extracellular levels only in repeated stress rats. Consistently, cocaine was able to increase VTA DA extracellular levels in repeated stress rats when 100 mu M bicuculline, an antagonist of GABA(A) receptor, was perfused intra VTA. Thus, both CRF and GABA systems are involved in the lack of response to cocaine in the VTA of repeated stress rats. It is tempting to suggest that the loss of response in VTA dopaminergic neurons to cocaine, after repeated stress, is due to an interaction between CRF and GABA systems. (c) 2015 Elsevier Ltd. All rights reserved. Keywords. Author Keywords:Cocaine; VTA; Stress; Dopamine . KeyWords Plus:CORTICOTROPIN-RELEASING-FACTOR; NUCLEUS-ACCUMBENS; INDUCED RELAPSE; LATERAL SEPTUM; RECEPTORS; CRF; TRANSMISSION; ADDICTION; GLUTAMATE; NEURONS
dc.descriptionRegular 2015
dc.descriptionFONDECYT
dc.descriptionFONDECYT
dc.languageeng
dc.relationhandle/10533/111557
dc.relationhandle/10533/111541
dc.relationhandle/10533/108045
dc.relationhttps://doi.org/10.1016/j.phrs.2015.08.015
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 Chile
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
dc.rightsinfo:eu-repo/semantics/article
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleExposure to repeated immobilization stress inhibits cocaine-induced increase in dopamine extracellular levels in the rat ventral tegmental area
dc.titlePharmacological Research
dc.typeArticulo
dc.typeinfo:eu-repo/semantics/publishedVersion


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