Authentication of commercial chilean mollusks using ribosomal internal transcribed spacer (its) as specie-specific dna marker

GAYANA;
GAYANA

dc.creatorGallardo-Escarate, Cristian
dc.creatorValenzuela-Munoz, Valentina
dc.creatorAguilera-Muñoz, Felipe
dc.date2017-04-27T18:52:54Z
dc.date2022-07-07T02:25:05Z
dc.date2017-04-27T18:52:54Z
dc.date2022-07-07T02:25:05Z
dc.date2008
dc.date.accessioned2023-08-23T00:30:29Z
dc.date.available2023-08-23T00:30:29Z
dc.identifier0
dc.identifierD06I1027
dc.identifierD06I1027
dc.identifierWOS:000262269100007
dc.identifier0717-652X
dc.identifierhttps://hdl.handle.net/10533/197779
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8355401
dc.descriptionContemporary world food safety standards have emphasized the implementation of efficient food traceability systems, including the correct labeling of products for the authentication of the commercialized species. In this context, Chile has reached an important development in agricultural exportations, reaching near US$ 3.82 billion in 2007. Since the identification based on morphologic and organoleptic characteristics is complex during the elaboration process of aquaculture products, molecular tools need to be developed in order to genetically trace aquaculture products. This study analyzed five species of Chilean commercial mollusks using molecular DNA markers. From the partial sequences of the ribosomal region ITS1-5.8SrDNA-ITS2, the specific primers for the internal transcribed spacers 1 and 2 (ITS1, ITS2) were designed. These primers in 8 fresh species of mollusks, 5 commercial presentations and 3 processed species, obtaining specie-specific band patterns in all cases for both ITS. Additionally, the primers are shown to be highly specific and of replicable amplification products, independently from the commercial presentation of the studied species. Genetically trace aquaculture products. The presence of fraud for species substitution between canned abalone (Haliotis) and loco (Concholepas) species is discussed.
dc.descriptionThis Study was financed by FONDEF Grant D0611027, D07/1085 and INNOVA07CT9 PDT79. The authors thank Dr. Miguel Angel del Rio-Portilla for his Suggestions and comments oil the original version of the manuscript.
dc.description2
dc.descriptionFONDEF
dc.descriptioncristian.gallardo@udec.cl
dc.descriptionFONDEF [D0611027, D07/1085, INNOVA07CT9 PDT79]
dc.description2
dc.descriptionFONDEF
dc.description72
dc.languageENG
dc.publisherEDICIONES UNIV, CONCEPCION
dc.relationinstname: Conicyt
dc.relationreponame: Repositorio Digital RI2.0
dc.relationinstname: Conicyt
dc.relationreponame: Repositorio Digital RI2.0
dc.relationinfo:eu-repo/grantAgreement/Fondef/D06I1027
dc.relationinfo:eu-repo/semantics/dataset/hdl.handle.net/10533/93477
dc.relationhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-65382008000200007&lng=en&nrm=iso&tlng=en
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleAuthentication of commercial chilean mollusks using ribosomal internal transcribed spacer (its) as specie-specific dna marker
dc.titleGAYANA
dc.titleGAYANA
dc.typeArticulo
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.coverageCONCEPCION


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