Articulo
Genomic characterization of saccharomyces cerevisiae strains isolated from wine-producingareas in south america
JOURNAL OF APPLIED MICROBIOLOGY (IMPRESA);
J. Appl. Microbiol.
Registro en:
0
D98I1037
D98I1037
WOS:000221329000030
1364-5072
Autor
LAVIN-ACEVEDO, JORGE
MARTINEZ-FERNANDEZ, CLAUDIO
GANGA-MUÑOZ, MARIA
GAC, S.
Institución
Resumen
Aims: The wide use of yeast inoculum for wine fermentations permit the spreading of commercial Saccharomyces strains in wine areas all over the world. To study the impact of this practice on the autochthonous yeast populations it is necessary to have tools that permit the evaluation of the geographical origin of native isolates and differentiate them from commercial strains. Methods and Results: Electrophoretic karyotyping and mitochondrial DNA restriction analysis were used to characterize the genome of native S. cerevisiae isolates associated to wine from three countries in South America. Both methods revealed differences in the genomic structure between these populations, in addition to differences between sub-populations collected in wine-producing areas in Chile. Conclusions: Our data support that molecular polymorphism analysis may be useful to evaluate the geographical origin of native isolates of yeast strains for industrial use. Furthermore, these findings are in agreement with the idea of a clonal mode of reproduction of wine yeasts in natural environments. Significance and Impact of the Study: This study permits the characterization of native yeast isolates in relation to their geographical origin. This procedure could be used as a tool for evaluating if a native isolate derives from the realon were it was collected or if it is a strain derived from a commercial strain by microevolution. 0 27 FONDEF cmartinc@usach.cl 0 FONDEF 96