Articulo
Heterologous expression of a novel psychrophilic cu/zn superoxide dismutase from deschampsia antarctica
PROCESS BIOCHEMISTRY;
Process Biochem.
Registro en:
0
D03I1079
D03I1079
WOS:000268918300004
1359-5113
Autor
GUTIERREZ-MORAGA, ANA
GIDEKEL-BLUFSTEIN, MANUEL
BARBA-DE LA ROSA, ANA
DELEON-RODRIGUEZ, ANTONIO
GARCIA-ECHAURI, SERGIO
ORDOÑEZ, LEANDRO
ROJAS-CONTRERAS, JUAN
Institución
Resumen
Superoxide dismutase (SOD) catalyzes the conversion of the superoxide radical ((center dot)O(2)(-)) into oxygen and hydrogen peroxide. Deschampsia antarctica is a plant that grows in Antarctica and survives to extreme low temperature and high UV radiation, thus it is an ideal model to study novel antioxidants. A cDNA Cu/Zn-SOD gene from D. antarctica was cloned into a pET vector and expressed in Escherichia coli BL21-SI. 112 mg/L of recombinant Cu/Zn-SOD was attained in batch cultures in bioreactor. Using Ni-affinity gel chromatography, the recombinant Cu/Zn-SOD was recovered with a purity of 90% and a specific enzyme activity of 749 at 25 degrees C. However, zymogram test showed that the enzyme has more activity at 4 degrees C. This D. antarctica SOD could be used to reduce the oxidation of refrigerated and frozen foods. (C) 2009 Elsevier Ltd. All rights reserved. This work was partially financed by the grant Antarctica Des S-3607, DIUFRO D109-0081, D108-2002, FONDEF Grant D031-1079 and CONACyT Grant 82010. A. De Leon Rodriguez thanks to CONACyT for the sabbatical fellowship 79973 and Sergio A. Garcia thanks to CONACyT for his scholarship 209658. We thank to the Chilean Antarctic Institute (INACH) for the logistic support during the stay in the Scientific Base "Prof. Julio Escudero", King George Island, Antarctic. 5 FONDEF aleonr@ipicyt.edu.mx Antarctica Des [S-3607]; DIUFRO [D109-0081, D108-2002]; FONDEF [D031-1079]; CONACyT [82010, 79973, 209658] 9 FONDEF 44