Articulo
Enzymatic synthesis of fructooligosaccharides with high 1-kestose concentrations using response surface methodology
BIORESOURCE TECHNOLOGY;
Bioresour. Technol.
Registro en:
0
D07I1045
D07I1045
WOS:000297657400003
0960-8524
Autor
ZUÑIGA-HANSEN, MARIA ELVIRA
VEGA PAULINO
ROBERTO J.
Institución
Resumen
Response surface methodology was used as an optimization tool for the production of short chain fructooligosaccharides (sc-FOS) using the commercial cellulolytic enzyme preparation, Rohapect CM. Three independent variables, temperature, concentrations of sucrose and enzyme were tested in the reaction medium. The responses of the design were, yield (g sc-FOS/100 g initial sucrose), 1-kestose (g/100 g sc-FOS) and volumetric productivity (g sc-FOS/L h). Significant effects on the three responses included a quadratic effect (temperature), a linear effect (sucrose and enzyme concentrations) and an interaction between temperature and sucrose concentration. The cost-effective conditions to support the process in a high competitive market were 50 degrees C, 6.6 TU/mL enzyme, 2.103 M sucrose in 50 mM acetate buffer at pH 5.5, and the synthesis for a 5 h reaction time. Under these conditions, a high Y(P/S) (63.8%), Q(P) (91.9 g/L h) and sGF2 (68.2%) was achieved. (C) 2011 Elsevier Ltd. All rights reserved. This research was financially supported by the Project FONDEF DO7I1045 of Chile and the CREAS. In addition, we acknowledge the financial support (scholarship) of CONICYT for our PhD student, R.Vega. We are grateful to Matias Berndt (Dimerco Comercial Ltda., Chile) for the enzyme sample. 8 FONDEF mzuniga@ucv.cl FONDEF of Chile [DO7I1045]; CREAS; CONICYT FONDEF