Articulo
Volatile profiles of human skin cell cultures in different degrees of senescence
JOURNAL OF CHROMATOGRAPHY. B (PRINT);
J. Chromatogr. B
Registro en:
0
D02I1009
D02I1009
WOS:000274414500024
1570-0232
Autor
YOUNG-ANZE, MANUEL
ACEVEDO-GUTIERREZ, CRISTIAN
SANCHEZ-MONTIEL, ELIZABETTH
REYES-MARTINEZ, JUAN GUILLERMO
Institución
Resumen
It is known that skin releases volatile organic Compounds to the environment. and also that its emission pattern changes with aging of the skin. It could be considered, that these compounds are intermediaries in cell metabolism, since many intermediaries of metabolic pathways have a volatile potential. In this work, a simple and non-destructive method consisting of SPME sampling and GC/MS analysis was developed to identify volatile organic emanations from cell Cultures. This technique, applied to skin cells culture, indicates that the cells or cell metabolism produce several skin emissions. Chemometric analysis was performed in order to explore the relationship between a volatile profile and the senescence of cell cultures. Volatile profiles were different for cell cultures in different degrees of senescence. indicating that volatile compound patterns could be used to provide information about the age of skin cells. (C) 2009 Elsevier B.V. All rights reserved. The authors wish to thank CONICYT from Chile by: FONDEF Grant (# D0211009) and Doctoral Scholarship for Cristian Acevedo (# D-21050588). 8 FONDEF cristian.acevedo@usm.cl FONDEF [D0211009]; Doctoral Scholarship for Cristian Acevedo [D-21050588] 4 FONDEF 878