Conformational dynamics in TRPV1 channels reported by an encoded coumarin amino acid

ELIFE

dc.creatorSteinberg, Ximena
dc.creatorKasimova, Marina A
dc.creatorCabezas-Bratesco, Deny
dc.creatorGalpin, Jason D
dc.creatorLadron-de-Guevara, Ernesto
dc.creatorVilla, Federica
dc.creatorCarnevale, Vincenzo
dc.creatorIslas, León
dc.creatorAhern, Christopher A
dc.creatorBrauchi-Ulloa, Sebastián Esteban
dc.date2021-08-23T22:57:13Z
dc.date2022-07-07T02:39:21Z
dc.date2021-08-23T22:57:13Z
dc.date2022-07-07T02:39:21Z
dc.date2017
dc.date.accessioned2023-08-22T01:31:00Z
dc.date.available2023-08-22T01:31:00Z
dc.identifier1151430
dc.identifier1151430
dc.identifierhttps://hdl.handle.net/10533/251992
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8306253
dc.descriptionTRPV1 channels support the detection of noxious and nociceptive input. Currently available functional and structural data suggest that TRPV1 channels have two gates within their permeation pathway: one formed by a 'bundle-crossing' at the intracellular entrance and a second constriction at the selectivity filter. To describe conformational changes associated with channel gating, the fluorescent non-canonical amino acid coumarin-tyrosine was genetically encoded at Y671, a residue proximal to the selectivity filter. Total internal reflection fluorescence microscopy was performed to image the conformational dynamics of the channels in live cells. Photon counts and optical fluctuations from coumarin encoded within TRPV1 tetramers correlates with channel activation by capsaicin, providing an optical marker of conformational dynamics at the selectivity filter. In agreement with the fluorescence data, molecular dynamics simulations display alternating solvent exposure of Y671 in the closed and open states. Overall, the data point to a dynamic selectivity filter that may serve as a gate for permeation.
dc.descriptionRegular 2015
dc.descriptionFONDECYT
dc.descriptionFONDECYT
dc.languageeng
dc.relationhandle/10533/111557
dc.relationhandle/10533/111541
dc.relationhandle/10533/108045
dc.relationhttps://doi.org/10.7554/eLife.28626
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 Chile
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
dc.rightsinfo:eu-repo/semantics/article
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleConformational dynamics in TRPV1 channels reported by an encoded coumarin amino acid
dc.titleELIFE
dc.typeArticulo
dc.typeinfo:eu-repo/semantics/publishedVersion


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