Articulo
Development and characterization of est-microsatellites for the edible sea urchin loxechinus albus using next generation sequencing
CONSERVATION GENETICS RESOURCES;
Conserv. Genet. Resour.
Registro en:
1877-7260
D09I1065
D09I1065
WOS:000335959600047
1877-7252
Autor
Arevalo, Alejandra
Bruning, Paulina
Sanchez, Roland
Cardenas, Leyla
Institución
Resumen
The Loxechinus albus is the only sea urchin exploited for commercial fisheries and is an important component of communities across southeastern Pacific coast. Despite its ecological and economic importance, limited information is known about genetic diversity and the actual connectivity among their populations. From 43,608 contigs obtained from their transcriptome we identified in silico a total of 463 putative microsatellite loci. From here, 17 expressed sequence tag-derived simple sequence repeats were developed. The number of alleles per locus ranged from 1 to 18 and no significant linkage disequilibrium was detected between locus pairs. Deviations for Hardy-Weinberg equilibrium were observed only in 6 of 17 loci in one location. These new markers will be useful to assess the levels of genetic connectivity among populations. Authors acknowledge to Dr. Carlos Molinet for collecting samples along the Chilean coast. The study was financial supported by Fondo de Fomento al Desarrollo Cientifico y Tecnologico FONDEF D09I1065. Alejandra Arevalo was supported by a doctoral fellowship 24121486, CONICYT-Chile. 0 FONDEF leylacardenas1@gmail.com Fondo de Fomento al Desarrollo Cientifico y Tecnologico FONDEF [D09I1065]; CONICYT-Chile [24121486] FONDEF