Most research in physiological ecology has focused on the effects of mean changes in temperature under the classic "hot vs cold" acclimation treatmenthowever, current evidence suggests that an increment in both the mean and variance of temperature could act synergistically to amplify the negative effects of global temperature increase and how it would affect fitness and performance-related traits in ectothermic organisms. We assessed the effects of acclimation to daily variance of temperature on thermal performance curves of swimming speed in helmeted water toad tadpoles (Calyptocephalella gayi). Acclimation treatments were 20 degrees C +/- 0.1 SD (constant) and 20 degrees C +/- 1.5 SD (fluctuating). We draw two key findings: first, tadpoles exposed to daily temperature fluctuation had reduced maximal performance (Z(max)), and flattened thermal performance curves, thus supporting the "vertical shift or faster-slower" hypothesis, and suggesting that overall swimming performance would be lower through an examination of temperatures under more realistic and ecologically-relevant fluctuating regimenssecond, there was significant interindividual variation in performance traits by means of significant repeatability estimates. Our present results suggest that the widespread use of constant acclimation temperatures in laboratory experiments to estimate thermal performance curves (TPCs) may lead to an overestimation of actual organismal performance. We encourage the use of temperature fluctuation acclimation treatments to better understand the variability of physiological traits, which predict ecological and evolutionary responses to global change.Author Keywords:AcclimationThermal performance curveTPCsDaily thermal fluctuationInterindividual variationTadpole swimming performanceCalyptocephalella gayiRegular 2015FONDECYTFONDECYT