Articulo
Coumarin-based fluorescent probes for dual recognition of copper(ii) and iron(iii) ions and their application in bio-imaging
SENSORS;
Sensors
Registro en:
0
VIU110063
VIU110063
WOS:000336039100075
1424-8220
Autor
Garcia-Beltran, Olimpo
Cassels, Bruce K.
Perez, Claudio
Mena, Natalia
Nunez, Marco T.
Martinez, Natalia P.
Pavez, Paulina
Aliaga, Margarita E.
Institución
Resumen
Two new coumarin-based "turn-off" fluorescent probes, (E)-3-((3,4-dihydroxybenzylidene)amino)-7-hydroxy-2H-chromen-2-one (BS1) and (E)-3-((2,4-dihydroxybenzylidene)amino)-7-hydroxy-2H-chromen-2-one (BS2), were synthesized and their detection of copper(II) and iron(III) ions was studied. Results show that both compounds are highly selective for Cu2+ and Fe3+ ions over other metal ions. However, BS2 is detected directly, while detection of BS1 involves a hydrolysis reaction to regenerate 3-amino-7-hydroxycoumarin (3) and 3,4-dihydroxybenzaldehyde, of which 3 is able to react with copper(II) or iron(III) ions. The interaction between the tested compounds and copper or iron ions is associated with a large fluorescence decrease, showing detection limits of ca. 10(-5) M. Preliminary studies employing epifluorescence microscopy demonstrate that Cu2+ and Fe3+ ions can be imaged in human neuroblastoma SH-SY5Y cells treated with the tested probes. This work was supported by FONDECYT Grant #1130062 and by FONDEF VIU-110063. 10 FONDEF ojgarciab@ug.uchile.cl; bcassels@u.uchile.cl; claudio.perez.mendez@gmail.com; npaz81@hotmail.com; mnunez@uchile.cl; natalia.dpma@gmail.com; ppavezg@uc.cl; mealiaga@uc.cl FONDECYT [1130062]; FONDEF [VIU-110063] FONDEF