Localization and function of Rhipicephalus (Boophilus) microplus vitellin-degrading cysteine endopeptidase

dc.creatorSeixas, Adriana
dc.creatorEstrela, Andreia Bergamo
dc.creatorCeolato, Juliana Casagrande
dc.creatorPontes, Emerson Guedes
dc.creatorLara, Flávio Alves
dc.creatorGondim, Katia Calp
dc.creatorTermignoni, Carlos
dc.date2011-01-21T05:59:03Z
dc.date2010
dc.identifier0031-1820
dc.identifierhttp://hdl.handle.net/10183/27466
dc.identifier000758964
dc.descriptionThe tick Rhipicephalus (Boophilus) microplus is an important parasite of cattle in many areas of the tropics. Characterization of molecules involved in mechanisms such as vitellogenesis and embryo development may contribute to a better understanding of this parasite’s physiology. The vitellin-degrading cysteine endopeptidase (VTDCE) is the most active enzyme involved in vitellin hydrolysis in R. microplus eggs. Here we show an association between VTDCE and vitellin in an additional site, apart from the active site. Our data also demonstrate cysteine endopeptidase activity in different tissues such as ovary, gut, fat body, salivary gland and female haemolymph, where it is controlled by a physiological inhibitor. In R. microplus female gut, VTDCE is localized in areas of protein synthesis and trafficking with the underlying haemolymph. VTDCE is also localized in the ovary basal region, in vesicle membranes of ovary pedicel cells and in oocyte cytosol. These results suggest that VTDCE plays a role in vitellin digestion during tick development.
dc.formatapplication/pdf
dc.languageeng
dc.relationParasitology. Cambridge, United Kingdom. Vol. 137, no. 12 (Oct. 2010), p. 1819-1831
dc.rightsOpen Access
dc.subjectRhipicephalus microplus
dc.subjectCarrapatos
dc.subjectCisteína endopeptidases
dc.subjectBioquímica
dc.subjectRhipicephalus (Boophilus) microplus
dc.subjectCysteine endopeptidase
dc.subjectCysteine endopeptidase inhibitor
dc.subjectHaemolymph
dc.subjectVitellin
dc.subjectVTDCE
dc.titleLocalization and function of Rhipicephalus (Boophilus) microplus vitellin-degrading cysteine endopeptidase
dc.typeArtigo de periódico
dc.typeEstrangeiro


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