Para eliminar o disminuir los daños que los residuos sólidos urbanos provocan, se destaca el tratamiento mediante digestión anaeróbica como uno de los procesos más atractivos en la actualidad. Los problemas de control y estabilización de los sistemas convencionales de digestión anaerobia han llevado a nuevas soluciones tecnológicas, entre estas la separación de fases, que permite la optimización de la acidogénesis y metanogénesis por separado. La primera fase ha sido poco estudiada y en esta se obtienen, por fermentación, hidrógeno y ácidos grasos volátiles, cuyas aplicaciones hacen que el proceso de fermentación acidogénica tome importancia; presentándose como una plataforma que consolida el concepto de biorefinería en lugar de una etapa de tratamiento convencional. Este trabajo tiene el objetivo de realizar un estudio para la evaluación del proceso de digestión anaeróbica de la fracción orgánica de los residuos sólidos urbanos de Manta, en un proceso en fases separadas como parte de la alternativa de solución de los residuos sólidos urbanos. Mediante la metodología propuesta se realiza la simulación del proceso a nivel de laboratorio, cuyos resultados constituyen elementos importantes para la implementación del proceso. Se obtienen remociones promedio de Sólidos Totales de 58,24 % y 64,83 % de Sólidos Totales Volátiles y se presentan los rendimientos de la producción de hidrógeno y metano. La evaluación ambiental mediante análisis de ciclo de vida muestra que los impactos ambientales más significativos del mismo están en las categorías de agotamiento del ozono estratosférico, la eutrofización del agua fresca y escasez de recursos minerales. Palabras clave: Residuos sólidos urbanos, digestión anaeróbica, fases separadas, análisis de ciclo de vida. ABSTRACT To eliminate or reduce the damage caused by solid urban waste, treatment by anaerobic digestion stands out as one of the most attractive processes today. The control and stabilization problems of conventional anaerobic digestion systems have led to new technological solutions, among them phase separation, which allows the optimization of acidogenesis and methanogenesis separately. The first phase has been little studied and in this phase, hydrogen and volatile fatty acids are obtained by fermentation, whose applications make the acidogenic fermentation process become important; presenting itself as a platform that consolidates the biorefinery concept instead of a conventional treatment stage. This work aims to carry out a study for the evaluation of the anaerobic digestion process of the organic fraction of urban solid waste from Manta, in a process in separate phases as part of the alternative solution for urban solid waste. Through the proposed methodology, the simulation of the process is carried out at the laboratory level, the results of which constitute important elements for the implementation of the process. Average removals of Total Solids of 58.24% and 64.83% of Total Volatile Solids are obtained and the yields of hydrogen and methane production are presented. The environmental assessment through life cycle analysis shows that the most significant environmental impacts are in the categories of stratospheric ozone depletion, eutrophication of fresh water and scarcity of mineral resources. Keywords: Urban solid waste, anaerobic digestion, separate phases, life cycle analysis. Información del manuscrito: Fecha de recepción: 20 de mayo de 2020 Fecha de aceptación: 08 de julio de 2020 Fecha de publicación: 09 de julio de 2020To eliminate or reduce the damage caused by solid urban waste, treatment by anaerobic digestion stands out as one of the most attractive processes today. The control and stabilization problems of conventional anaerobic digestion systems have led to new technological solutions, among them phase separation, which allows the optimization of acidogenesis and methanogenesis separately. The first phase has been little studied and in this phase, hydrogen and volatile fatty acids are obtained by fermentation, whose applications make the acidogenic fermentation process become important; presenting itself as a platform that consolidates the biorefinery concept instead of a conventional treatment stage. This work aims to carry out a study for the evaluation of the anaerobic digestion process of the organic fraction of urban solid waste from Manta, in a process in separate phases as part of the alternative solution for urban solid waste. Through the proposed methodology, the simulation of the process is carried out at the laboratory level, the results of which constitute important elements for the implementation of the process. Average removals of Total Solids of 58.24% and 64.83% of Total Volatile Solids are obtained and the yields of hydrogen and methane production are presented. The environmental assessment through life cycle analysis shows that the most significant environmental impacts are in the categories of stratospheric ozone depletion, eutrophication of fresh water and scarcity of mineral resources. Keywords: Urban solid waste, anaerobic digestion, separate phases, life cycle analysis.