In this work bacterial strains able to degrade hydrocarbons were isolated and identified, from samples taken from the following locations: Papallacta, Sacha Central and Pozo Guanta 10, where oil spills had occurred. The first step was a bacterial rescue with LB (Luria-Bertulli) broth, after this those bacteria were isolated in LB agar, where consecutive isolation was made to achieve pure growth. Those isolated bacteria were put under hexane steam, a hydrocarbon of high bactericidal effect, to assure bacterial degradation process.En este trabajo se aisló e identificó cepas bacterianas capaces de degradar hidrocarburos derivados del petróleo, en muestras de Papallacta, Sacha Central y del Pozo Guanta 10, lugares donde se dieron derrames. De las muestras tomadas se hizo un rescate bacteriano haciendo un enriquecimiento con caldo de cultivo LB (Luria-Bertulli), para luego sembrar por extensión en placa en agar LB. Después con estriaciones consecutivas en agar LB se logró aislar cepas bacterianas a las que se sometió a vapores de hexano, como método de selección y así asegurar presenten una actividad degradadora de hidrocarburos.