Article
Culture supernatants of cervical cancer cells induce an M2 phenotypic profile in THP-1 macrophages
Registro en:
Pedraza Brindis E.J., SánchezReyes K., Hernández Flores G., Bravo Cuellar A., Jave Suárez L.F., Aguilar Lemarroy A., Gómez Lomelí P., López López B.A. y Ortiz Lazareno P.C. (2016) Culture supernatants of cervical cancer cells induce an M2 phenotypic profile in THP-1 macrophages. Cellular Immunology, Vol. 310, pp: 42-52
0008-8749
Autor
Pedraza Brindis, Eliza Julia
Sánchez Reyes, Karina
Hernández Flores, Georgina
Bravo Cuéllar, Alejandro
Jave Suárez, Luis Felipe
Aguilar Lemarroy, Adriana
Gómez Lomelí, Paulina
López López, Brenda Anahí
Ortiz Lazareno, Pablo Cesar
Institución
Resumen
Patients with cervical cancer (CxCa) typically present an infiltrate of tumor-associated macrophages, which is associated with a poor prognosis. We found that CxCa cell lines (HeLa, SiHa, and C-33A) secreted factors involved in regulating tumor growth including IL-6, IL-4, PDGFAA, HGF, VEGF, ANG-2, and TGF-β3. We assessed the effects of culture supernatants from these cell lines on macrophages derived from the THP-1 cell line. Macrophages treated with culture supernatants from CxCa cells developed an M2-like phenotype with expression of CD163, low nitric oxide release, and high secretion of IL-6, PDGFAA, HGF, ANG-2, and VEGF. The macrophages continued to produce PDGFAA, PDGFBB, and VEGF 48 h after the CxCa cell culture supernatants were removed. The induction of M2 macrophages in vivo favors tumor growth, angiogenesis, tissue remodeling, and metastasis. These results demonstrated that factors secreted by CxCa cells induced a stable M2 phenotype in THP-1 macrophages. División de Inmunología, Centro de Investigación Biomédica de Occidente (CIBO), Instituto Mexicano del Seguro Social (IMSS), Guadalajara, Jalisco, México
Programa de Doctorado en Ciencias Biomédicas Orientación Inmunología, Centro Universitario de Ciencias de la Salud (CUCS), Universidad de Guadalajara (UdeG), Jalisco, Mexico
Departamento de Ciencias de la Salud, Centro Universitario de los Altos, UdeG, Tepatitlán de Morelos, Jalisco, México
Universidad Autónoma de Guadalajara, Zapopan, Jalisco, México