info:eu-repo/semantics/article
Identification and quantification of a phytotoxic metabolite from Alternaria dauci
Autor
Martha Leyte-Lugo
Pascal Richomme
Pascal Poupard
Luis Manuel Peña Rodríguez
Resumen
Alternaria dauci is the causal agent of Alternaria leaf blight (ALB) in carrot (Daucus carota) crops around the world. However, to date, A. dauci has received limited attention in its production of phytotoxic metabolites. In this investigation, the bioassay-guided isolation of the extract from liquid cultures of A. dauci resulted in the isolation of two metabolites identified as α-acetylorcinol (1) and p-hydroxybenzoic acid (2), based on their spectroscopic data and results from chemical correlation reactions. Testing of both metabolites in different assays showed an important phytotoxic activity for p-hydroxybenzoic acid (2) when tested in the leaf-spot assay on parsley (Petroselinum crispum), in the leaf infiltration assay on tobacco (Nicotiana alata) and marigold (Tagetes erecta), and in the immersion assay on parsley and parsnip (Pastinaca sativa) leaves. Quantification of the two metabolites in the crude extract of A. dauci kept at different times showed that p-hydroxybenzoic acid (2) is one of the first metabolites to be synthesized by the pathogen, suggesting that this salicylic acid derivative could play an important role in the pathogenicity of the fungus.
Materias
Ítems relacionados
Mostrando ítems relacionados por Título, autor o materia.
-
Preparación de materiales compuestos mediante la funcionalización covalente de nanotubos de carbono (CNTs) con polímero
CLAUDIA GUADALUPE ESPINOSA GONZALEZ -
Attachment of transition metal nanoparticles on nitrogen doped carbon nanotubes (MWNTs-CNx) and their further reactions.
XAVIER NORBERTO LEPRÓ CHÁVEZ -
Alternativas sustentables para uso de residuos de explotación de tobas volcánicas en materiales de construcción
REBECA GUADALUPE ORTIZ MENA; MARIA GUADALUPE NAVARRO ROJERO; T. LOPEZ-LARA; Juan Bosco Hernández Zaragoza; DANIEL NAPOLEON GOMEZ BALBUENA