Agrobacterium tumefaciens-mediated transformation of common bean (Phaseolus vulgaris) var. Brunca

dc.creatorLAURA YESENIA SOLÍS RAMOS
dc.creatorJulio César Ortiz Pavón
dc.creatorANTONIO ANDRADE TORRES
dc.creatorRomano Porras Murillo
dc.creatorArturo Brenes Angulo
dc.creatorEnrique Castaño de la Serna
dc.date2019
dc.date.accessioned2023-07-21T19:18:57Z
dc.date.available2023-07-21T19:18:57Z
dc.identifierhttp://cicy.repositorioinstitucional.mx/jspui/handle/1003/1689
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7737249
dc.descriptionCommon bean is a crop recalcitrant to in vitro regeneration and therefore it lacks an efficient transformation protocol that can be reproduced using A. tumefaciens. The main goal of this study was to establish a protocol for A. tumefaciens mediated transformation of Phaseolus vulgaris var. Brunca by marker genes (gusA and nptII) together with the gene for trehalose-6-phosphate synthase from Saccharomyces cerevisiae (TPS1) used in other species to increase tolerance to abiotic stress. The β-glucuronidase activity was detected in 45 % of the LBA4404 ElectroMAX® pCAMBIA1301 infected explants. Transformed explants regenerated new shoots after four to five months period in a kanamycin rich media. Surviving plants were evaluated by PCR and presented an 0.5 % efficiency of transformation. The established protocol for genetic transformation of common bean has two additional advantages with respect to previous reports: (1) it allows for obtaining transformed regenerants and (2) the genetic transformation was stable for the selective gene.
dc.formatapplication/pdf
dc.languageeng
dc.relationinfo:eu-repo/semantics/datasetDOI/10.15517/RBT.V67I2SUPL.37208
dc.relationcitation:Solís-Ramos, L. Y., Ortiz-Pavón, J. C., Andrade-Torres, A., Porras-Murillo, R., Brenes-Angulo, A., & de la Serna, E. C. (2019). Agrobacterium tumefaciens-mediated transformation of common bean (Phaseolus vulgaris) var. Brunca. Revista de Biología Tropical, 67(2 SUPL), S83-S94
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/4.0
dc.sourceRevista de Biología Tropical, 67(2 SUPL), S83-S94
dc.subjectinfo:eu-repo/classification/Autores/COMMON BEAN
dc.subjectinfo:eu-repo/classification/Autores/RECALCITRANT SPECIES
dc.subjectinfo:eu-repo/classification/Autores/GENETIC TRANSFORMATION
dc.subjectinfo:eu-repo/classification/Autores/A. TUMEFACIENS
dc.subjectinfo:eu-repo/classification/Autores/GUSA ACTIVIT
dc.subjectinfo:eu-repo/classification/cti/2
dc.subjectinfo:eu-repo/classification/cti/24
dc.subjectinfo:eu-repo/classification/cti/2415
dc.subjectinfo:eu-repo/classification/cti/241502
dc.subjectinfo:eu-repo/classification/cti/241502
dc.titleAgrobacterium tumefaciens-mediated transformation of common bean (Phaseolus vulgaris) var. Brunca
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion


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