info:eu-repo/semantics/article
Agrobacterium tumefaciens-mediated transformation of common bean (Phaseolus vulgaris) var. Brunca
Autor
LAURA YESENIA SOLÍS RAMOS
Julio César Ortiz Pavón
ANTONIO ANDRADE TORRES
Romano Porras Murillo
Arturo Brenes Angulo
Enrique Castaño de la Serna
Resumen
Common bean is a crop recalcitrant to in vitro regeneration and therefore it lacks an efficient transformation protocol that can be reproduced using A. tumefaciens. The main goal of this study was to establish a protocol for A. tumefaciens mediated transformation of Phaseolus vulgaris var. Brunca by marker genes (gusA and nptII) together with the gene for trehalose-6-phosphate synthase from Saccharomyces cerevisiae (TPS1) used in other species to increase tolerance to abiotic stress. The β-glucuronidase activity was detected in 45 % of the LBA4404 ElectroMAX® pCAMBIA1301 infected explants. Transformed explants regenerated new shoots after four to five months period in a kanamycin rich media. Surviving plants were evaluated by PCR and presented an 0.5 % efficiency of transformation. The established protocol for genetic transformation of common bean has two additional advantages with respect to previous reports: (1) it allows for obtaining transformed regenerants and (2) the genetic transformation was stable for the selective gene.
Materias
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