info:eu-repo/semantics/article
Plant regeneration of Mullein Nightshade (Solanum donianum Walp.) from leaf explants
Autor
INGRID AILEEN O'CONNOR SANCHEZ
Resumen
A reliable protocol for plant regeneration of Mullein
Nightshade (Solanum donianum Walp.) was developed from in vitro
leaf explants. They were cultured on Murashige and Skoog semisolid
medium, supplemented with several combinations of zeatin riboside
and 1-naphthaleneacetic acid for shoot regeneration. The formulation
that
originated
most
shoots
per
explant
was
8.5
μM
zeatin
riboside
in
absence
of
1-naphthalene
acetic
acid.
Explants
with
shoots
were
transferred to Murashige and Skoog medium, with half the
normal salt concentration and without plant growth regulators for
elongation. Elongated shoots were individualized, and they rooted
readily in half-strength Murashige and Skoog medium without plant
growth regulators. The regenerated plants, which were transferred to
soil in a greenhouse, followed a similar phenological pattern to plants
grown from seed.