info:eu-repo/semantics/article
HUVEC biocompatibility and platelet activation of segmented polyurethanes prepared with either glutathione or its amino acids as chain extenders
Autor
JUAN VALERIO CAUICH RODRIGUEZ
Resumen
Novel biodegradable segmented polyurethanes (SPUs) were synthesized with polycaprolactone
diol, 4,4′-methylen bis (cyclohexyl isocyanate) and either L-glutathione or its
constituent amino acids (L-glutamic acid, L-cysteine and glycine) as chain extenders.
Fourier transform infrared spectroscopy analysis revealed the feasibility of obtaining polyurethanes
through the presence of NH (Amide II), C–N, C–O, and C=O bands and the
absence of NCO band. Differential scanning calorimetry and X-ray diffraction revealed that
a semicrystalline polymer (Tm = 42–52 °C; 2h = 21.3° and 23°) was obtained in all cases,
while dynamic mechanical analysis (DMA) revealed an amorphous phase (Tg = 30 to
36 o
C). These properties, in addition to their high molecular weight, led to high moduli
and higher extensibilities when glycine and glutamic acid were used as chain extenders.
Clotting times (Lee–White test) and activated partial thromboplastin time determined on
these polyurethanes were longer than with glass. In addition, all synthesized SPU exhibited
platelet activation indexes below the collagen type I positive control. Human umbilical vein
endothelial cells viability was higher in SPUs containing either glycine or cysteine. The
obtained results indicate that SPUs that use cysteine as chain extender are promising
candidates for cardiovascular applications.