Spain An automatic system based on multisyringe flow injection analysis (MSFIA) and lab-on-valve (LOV) techniques for separation and pre-concentration of 226Ra in drinking water samples has been developed. The analytical protocol combines two different procedures for radium separation and pre-concentration: the Ra adsorption on MnO2 and the BaSO4 co-precipitation, achieving more selectivity especially in water samples with low radiumlevels. Radium is adsorbed on MnO2 deposited on macroporous of bead cellulose. Then, it is eluted with hydroxylamine and subsequently co-precipitated with BaSO4. After that, the remaining liquid is discarded. The 226Ra pre-concentrated in the solid can be detected in off-line mode by low background proportional counter or if the co-precipitate is dissolved using EDTA in ammonium solution. 226Ra activity is determined using the Hidex 300SL liquid scintillation counter (LSC). The MSFIA-LOV system allows reduction of reagent volumes, the minimization of sample handling, improving the precision (1.9%RSD), and extraction frequency (up to 3 h-1), attaining a significant decrease of both time and cost per analysis. Besides, it has been satisfactorily applied to different types of spiked drinking water (tap, mineral and groundwater). The minimum detectable activity (AMD) of this systemallows to reach the reference values proposed by international agencies responsible for providing technical support on public health issues (e.g.WHO, ATSDR, EPA).